LOCUS       Exported                 876 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  This red fluorescent plasma membrane marker consists of the first 
            twenty residues of neuromodulin fused to mCherry. .
ACCESSION   .
VERSION     .
KEYWORDS    mCherry-Mem
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    16..96
                     /label=MCS
                     /note="multiple cloning site"
     CDS             164..874
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALR
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(309..327)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     577..596
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct
       61 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatgctgt gctgtatgag
      121 aagaaccaaa caggttgaaa agaatgatga ggaccaaaag atcatggtga gcaagggcga
      181 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      241 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      301 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      361 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      421 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      481 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      541 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      601 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga ggggcgagat
      661 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      721 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      781 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      841 ctccaccggc ggcatggacg agctgtacaa gtaagc
//
