LOCUS       Exported                2690 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Universal domesticator plasmid for level 0 parts.
ACCESSION   .
VERSION     .
KEYWORDS    pUPD2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    82..103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        118..148
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    156..172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     161..183
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     180..196
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     180..196
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    209..265
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(266..283)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(266..282)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(275..297)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(491..510)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     terminator      647..704
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(728..745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(899..1487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(979..998)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1670..1764
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1785..2444)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2359..2378
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2606..2624
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2628..2671
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2674..5
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg tgagacgcag ctggcacgac aggtttcccg actggaaagc
       61 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      121 acactatatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      181 aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg
      241 atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      301 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      361 atagcgaaga ggcctgcgcc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      421 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
      481 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
      541 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
      601 tgcgtctcat gagaccactc atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg
      661 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
      721 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      901 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1321 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1501 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1561 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1621 caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa
     1681 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     1741 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
     1801 cactcgtcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     1861 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     1921 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     1981 ctggtgaaac tcacccaggg attggctgat acgaaaaaca tattctcaat aaacccttta
     2041 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     2101 tgccggaaat cgtcgtggta ttcactccag agtgatgaaa acgtttcagt ttgctcatgg
     2161 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     2221 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     2281 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     2341 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     2401 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     2461 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     2521 aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat
     2581 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     2641 aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc
//
