LOCUS Exported 3362 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pLexA::SRDX (GB0732) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3362) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 3362) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3362 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 6..257 /codon_start=1 /gene="lexA (truncated)" /product="DNA-binding domain of E. coli LexA" /label=LexA BD /note="binds the 16-bp palindromic sequence 5'-CTGTATATATATACAG-3'" /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA" promoter complement(413..431) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(414..431) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(449..465) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(449..465) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(462..484) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 473..489 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(497..527) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 542..563 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(680..697) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(851..1439) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(931..950) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1610..2470) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGPRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2233..2252 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2471..2575) /gene="bla" /label=AmpR promoter rep_origin complement(2653..3108) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2790..2811) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3002..3021 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3234..3256 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3248..3265 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3249..3265 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3272..3291 /label=T7 /note="T7 promoter, forward primer" promoter 3272..3290 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3321..3330 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3325..3334 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgaatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca 61 tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt 121 ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgtt attgaaattg 181 tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg ttgccgctgg 241 taggtcgtgt ggctgccggt gaaccggagc cacttgatct tgatcttgaa cttagacttg 301 gttttgctta ggcttcgaga ccactcatcg cccatcacta gtgaattcgc ggccgcctgc 361 aggtcgacca tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt 421 gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 481 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 541 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 601 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 661 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 721 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 781 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 961 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 1021 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 1081 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 1141 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 1201 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 1261 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 1321 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 1381 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 1441 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 1501 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 1561 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 1621 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 1681 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 1741 tgataccgcg aggcccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 1801 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 1861 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 1921 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 1981 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 2041 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 2101 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 2161 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 2221 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 2281 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 2341 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 2401 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 2461 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 2521 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 2581 ttccccgaaa agtgccacct gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 2641 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 2701 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 2761 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 2821 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgctggc ccactacgtg 2881 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 2941 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 3001 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 3061 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt 3121 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 3181 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 3241 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg 3301 cccgacgtcg catgctcccg gccgccatgg cggccgcggg aattcgatgg gcgatgagtg 3361 gt //