LOCUS Exported 12018 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. ACCESSION . VERSION . KEYWORDS pEGB2alpha2 35s:hCas9:tNos (GB0639) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12018) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 12018) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12018 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 616..960 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 904..924 /label=35S promoter /note="CaMV 35S promoter, forward primer" CDS 1033..5136 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 5149..5169 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 5196..5448 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(5483..5501) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" CDS 6994..7623 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 8057..9124 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" rep_origin 9190..9384 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(9620..9642) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 9728..9868 /label=bom /note="basis of mobility region from pBR322" primer_bind 9783..9802 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(9883..9900) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10054..10642) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10134..10153) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10730..11545) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 11453..11472 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" misc_feature 11978..12000 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA (truncated)" ORIGIN 1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta 61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga 121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga 181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa 241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa 301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg 361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac 421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa 481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt 541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga 601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt 661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta 721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg 781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac 841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc 901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac 961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact 1021 attctagtcg aaatggacaa gaagtactcc attgggctcg atatcggcac aaacagcgtc 1081 ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc 1141 aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg 1201 gaaacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag 1261 aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct 1321 ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac 1381 ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat 1441 catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc 1501 gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca 1561 gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc 1621 gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg 1681 tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc 1741 ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc 1801 gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac 1861 aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg 1921 tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg 1981 ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag 2041 gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa 2101 aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt 2161 aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa 2221 gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg 2281 ggcgaactgc acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac 2341 agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc 2401 cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagactat cactccctgg 2461 aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact 2521 aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag 2581 tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag 2641 ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac 2701 cggaaagtta ccgtgaaaca gctcaaagaa gattatttca aaaagattga atgtttcgac 2761 tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat 2821 ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt 2881 gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg 2941 aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat 3001 acaggatggg ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga 3061 aagacaatcc tggattttct taagtccgat ggatttgcca accggaactt catgcagttg 3121 atccatgatg actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag 3181 ggggacagtc tccacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga 3241 atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc 3301 gagaatatcg ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac 3361 agtagggaaa ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt 3421 aaggaacacc cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg 3481 cagaacggca gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac 3541 gacgtggatc atatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg 3601 ttgacaagat ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc 3661 aagaaaatga aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag 3721 ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc 3781 atcaaaaggc agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat 3841 tcacgcatga acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt 3901 actctgaagt ctaagctggt ttcagatttc agaaaggact ttcagtttta taaggtgaga 3961 gagatcaaca attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca 4021 cttatcaaaa aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac 4081 gatgttagga aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac 4141 ttcttttaca gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag 4201 attcggaagc gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag 4261 ggtagggatt tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa 4321 aagaccgaag tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc 4381 gacaagctga tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct 4441 cctacagtcg cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa 4501 ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa 4561 aaccccatcg actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt 4621 aagcttccca agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt 4681 gcgggcgagc tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg 4741 tatctggcca gccactatga aaagctcaaa ggatctcccg aagataatga gcagaagcag 4801 ctgttcgtgg aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc 4861 tccaaaagag tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag 4921 cacagggata agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc 4981 aacttgggcg cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac 5041 acctctacaa aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat 5101 gaaacaagaa tcgacctctc tcagctcggt ggagacagca gggctgaccc caagaagaag 5161 aggaaggtgt gagcttggaa tggatcttcg atcccgatcg ttcaaacatt tggcaataaa 5221 gtttcttaag attgaatcct gttgccggtc ttgcgacgat tatcatataa tttctgttga 5281 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt 5341 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg 5401 caaactagga taaattatcg cgcdcggtgt catctatgtt actagatcgg gaattgccaa 5461 gctaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 5521 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 5581 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 5641 taaatgcttc aataatggga ccgactcgcg cttgagacga agcttgacgt aaacctaaga 5701 gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc 5761 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc 5821 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcatctgaaa 5881 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc 5941 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac 6001 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg 6061 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt 6121 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc 6181 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc 6241 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg 6301 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg 6361 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg 6421 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc 6481 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg 6541 gcgtgcaacg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg 6601 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg 6661 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga 6721 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga 6781 gccgcccgcg cacctctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc 6841 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa 6901 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatccg 6961 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag 7021 aaaggcgggt caggcaagac gaccatcgga acccatctag cccgcgccct gcaactcgcc 7081 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc 7141 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac 7201 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac 7261 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct 7321 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg 7381 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc 7441 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg 7501 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc 7561 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt 7621 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc 7681 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga 7741 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac 7801 gccaaggcaa gaccattacc gagctgctat ctgaatagat cgcgcagcta ccagagtaaa 7861 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat 7921 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg 7981 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc 8041 gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg 8101 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc 8161 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg 8221 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc 8281 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga 8341 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga 8401 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga 8461 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga 8521 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt 8581 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt 8641 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac 8701 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg 8761 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa 8821 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg 8881 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa 8941 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg 9001 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc 9061 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc 9121 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg 9181 actctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa 9241 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga 9301 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct 9361 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa 9421 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc 9481 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca 9541 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt 9601 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc 9661 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 9721 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 9781 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 9841 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 9901 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 9961 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 10021 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 10081 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 10141 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 10201 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 10261 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 10321 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 10381 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 10441 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 10501 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 10561 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 10621 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 10681 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtgat tagaaaaact 10741 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt 10801 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa 10861 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc 10921 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg 10981 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct 11041 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga 11101 gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc 11161 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata 11221 cctggaatgc tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac 11281 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca 11341 tctcatctgt aacaacattg gcaacgctac ctttgccatg tttcagaaac aactctggcg 11401 catcgggctt cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag 11461 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag 11521 acgtttcccg ttgaatatgg ctcataacag aacttattat ttccttcctc ttttctacag 11581 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt 11641 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg 11701 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc 11761 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca 11821 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa 11881 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg 11941 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa 12001 caagcttcgt ctcagtca //