LOCUS       Exported                 988 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Generate OCT4 mOrange reporter line without selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    OCT4-2A-mOrange
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Zhu Z, Verma N, Gonzalez F, Shi ZD, Huangfu D
  TITLE     A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human 
            Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 
            10.1016/j.stemcr.2015.04.016.
  PUBMED    26028531
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(815..831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(815..831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(828..850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    839..855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(863..893)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    908..929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttacagaga gaagttcgtg gcaccggtgc tagcgtttga atgcatggga gagcccagag
       61 tggtgacgga gacaggggga aaggcttccc cctcagggaa agggaccgag gagtacagtg
      121 cagtgaagtg agggctccca tagcctgggg taccaaaatg gggccctggg gccagaggaa
      181 aggacactgg tccccctgag aaaggagacc cagcagcctc aaaatcctct cgttgtgcat
      241 agtcgctgct tgatcgcttg cccttctggc gccggttaca gaaccacact cggaccacct
      301 gcaagtgaat gacagaaagg agaatgacat tagacaatga gctgagacgg gcctgactct
      361 gcttggacat tctatccaaa gccaacagcc ctagagcagt tagaggagga cattagagaa
      421 tgagctgaga caggcctgac tgcttggaca ttctgtccaa agccaacagc cctagagcag
      481 ttggaggagc cagagctagg gaaagcgagg tggtgacagg ggaaagagat ggagcccgca
      541 gagagacatg gcactcacat ccttctcgag cccaagctgc tgggcgatgt ggctgatctg
      601 ctgcagtgtg ggtttcgggc actgcaggaa caaattctcc aggttgcctc tcactcggtt
      661 ctcgatactg gttcgctttc tctttcgggc ctgcacgagg gtttctgctt tgcatatctg
      721 tgcaggtggg acctgcagga aaagggcgaa ttccagcaca ctggcggccg ttactagtgg
      781 atccgagctc ggtaccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      841 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      901 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      961 cgggaaacct gtcgtgccag ctgcatta
//
