LOCUS       Exported                 958 bp ds-DNA     linear   SYN 14-JUN-2021
DEFINITION  Mammalian expression of hArl4 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    pDSHA-ARL4-Myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 958)
  TITLE     Unpublished Kahn lab ARF plasmids
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(706..730)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(841..859)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(905..956)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtctc ttttcttttt ctgttgccgc aacatttttc
      121 ttcttttaat gatcatatca tgtagtttct caagtccttc ctttaggcca tctcctatga
      181 ttgcacaggt aggctgcaaa tgccaaggag ttgatgagct cagttcaccc attgctaaca
      241 atttctcaat ttctgaaagt gacaatgagt tcctcaaatc ttgtttgtta gcaactataa
      301 gtacagggac tccctgattt tctgatatcc tagttatttt gtgaagttca gttttggctt
      361 cttccatcct ttcgacatca acagagtcca caacaaatac aatgccatct gtgcatctgg
      421 tatatgactt ccacagtggc cttaatttct cctgaccacc tacatcccag aagtgaaaag
      481 tgactgtttt agaatttccc aaggttacct taattttctc agtgttaaat cctttggtag
      541 gtacggtatt tacaaattca ttgaactgca gcctgtataa gacagttgtc tttccagcac
      601 agtccaaacc cagaataaca atgtggaaag actgaaatga aggcaggttg gacaggatag
      661 aagtctggtc tgacagccca ttccccatgg ttctatctcc ttctaagcct gcttttttgt
      721 acaaacttgt ttgattcgag tgtcgacggt atcgataagc ttgatatcga attcctgcag
      781 cccgggggat ccgcccgggc tagagcggcc gccaccgcgg tggagctcca gcttttgttc
      841 cctttagtga gggttaattt cgagcttggc gtaatcgcta gcggatctga cggttcacta
      901 aaccagctct gcttatatag acctcccacg gtacacgcct acggcccatt tgcgtcaa
//
