LOCUS       Exported                 996 bp ds-DNA     linear   SYN 04-AUG-2021
DEFINITION  Mammalian expression of dmBACCS2NS, a modified Drosophila 
            melanogaster blue light-activated Ca2+ channel switch, and dOrai.
ACCESSION   .
VERSION     .
KEYWORDS    dmBACCS2NS-IRES-dOrai
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata 
            T
  TITLE     Light generation of intracellular Ca(2+) signals by a genetically 
            encoded protein BACCS.
  JOURNAL   Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021.
  PUBMED    26282514
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      606..615
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttagat cccgggcccg
       61 cggtaccgtc gacctagaca atgtccccgg atgcgccata gttcatgcca ttatttcgta
      121 tgttattgtg atgctccaaa tgatcctgct ccatctgctc cttgagcatc tccagctcac
      181 ggatgcccga gactgtcacc tcatatttgt gcgacaccag ggagcgatag aagtgaatgg
      241 cgaaggccat aaagatgatc atcaccggta tgaggaccac acacgctgac caagccgccg
      301 gcggacttag gtcatagaat ttcacccagc acaggatggc tatctccagt agaaagagta
      361 tgagtcccag cagcgtggag aatgcccacg ccgtttcaat gtaccaatgg agacgctcgt
      421 gtggcgattc gtggaccagg gaaatactat gcaaattaca caccgtctca atatttggca
      481 ggatacaggt gctgatcatc agggccagca tgtgcaccgc caccaggagg gtggtgcaga
      541 tggcgaaggc tatcagcatg cccggtggta cattcgtatc gtgatccagt tgcacctcca
      601 ccatcgccac catggcgaat ccggagagca gggcggacgt cttgctggat gcctttagct
      661 tggcccgact aagctggagc ttccgccagg acaggtaggt gggcgagtgg agatcctcgc
      721 cggattgcga catgctgctg ctggcggtgc gatggtgtcc gcgcggcttc gcctggttct
      781 gtgaggaagg gggcgggggc ggcgggaact gcagcagcga gttgctgatg ctggtgcgct
      841 ggaaacctgc gctgttgttg ctgttgctgc cgctacccgt gggactgttg ctgtggtgct
      901 gtggatgcgg atgcggatgt gcgtgcagcg ggaactgctg ctggaactga tgaccggtgg
      961 ccactgaagt ggcggctgca acggcggcgg ccacaa
//
