LOCUS Exported 3116 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Carries 3' UTR of CDK2AP1, a miR-21 target. ACCESSION . VERSION . KEYWORDS pDONR221_CDK2AP1_3UTR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3116) TITLE RNA-binding protein 3' UTR target plasmids (unpublished) JOURNAL Unpublished REFERENCE 2 (bases 1 to 3116) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3116 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1242..1341) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1357..1376) /label=T7 /note="T7 promoter, forward primer" promoter complement(1358..1376) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1381..1397) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1381..1397) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1499..1518) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1510..2319 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1577..1596) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2466..3054 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2955..2974 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggcttc ctcccctgtt cagatgaaac ccttgttttc aaaatggtta cagtttcgtt 721 tttcctccca tggttcactt ggctctgaac ctacagtctc aaagattgag aaaagatttt 781 gcagttaatt aggatttgca ttttaagtag ttaggaactg cccaggtttt ttttgttttt 841 taagcattga tttaaaagat gcacggaaag ttatcttaca gcaaactgta gtttgcctcc 901 aagacaccat tgtctccctt taatcttctc ttttgtatac atttgttacc catggtgttc 961 tttgttcctt ttcataagct aataccactg tagggatttt gttttgaacg catattgaca 1021 gcacgcttta cttagtagcc ggttcccatt tgccatacaa tgtaggttct gcttaatgta 1081 acttcttttt tgcttaagca tttgcatgac tattagtgct tcaaagtcaa tttttaaaaa 1141 tgcacaagtt ataaatacag aagaaagagc aacccaccaa acctaacaag gacccccgaa 1201 cactttcata ctaagactgt aagtagatct cagttctgcg cacccagctt tcttgtacaa 1261 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag 1321 tcaaaataaa atcattattg ccatccagct gatatcccct atagtgagtc gtattacatg 1381 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca 1441 caagataaaa taatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca 1501 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg 1561 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca 1621 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt 1681 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg 1741 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact 1801 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat 1861 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt 1921 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt 1981 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg 2041 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc 2101 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga 2161 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt 2221 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat 2281 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg 2341 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc 2401 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 2461 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2521 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 2581 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 2641 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2701 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2761 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2821 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2881 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2941 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 3001 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 3061 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt //