LOCUS       Exported                 720 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Mammalian expression of rat ErbB2 tagged with ECFP.
ACCESSION   .
VERSION     .
KEYWORDS    pErbB2-ECFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Lerdrup M, Hommelgaard AM, Grandal M, van Deurs B
  TITLE     Geldanamycin stimulates internalization of ErbB2 in a 
            proteasome-dependent way.
  JOURNAL   J Cell Sci. 2006 Jan 1;119(Pt 1):85-95. Epub 2005 Dec 13.
  PUBMED    16352662
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    127..248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(164..183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     218..237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(255..710)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(392..413)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     604..623
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
       61 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      121 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      241 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
      301 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      361 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
      421 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
      481 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
      541 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
      601 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
      661 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
//
