LOCUS       Exported                1008 bp ds-DNA     linear   SYN 15-JUN-2021
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    pCellFree_G03 CREBZF
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    173..197
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgcagggtac cacgcgtacc caacacacgg acacatgcac gaacacgcat gcagagagat
       61 catacatcat ctatagagaa gtacacgtaa aagtaagact cgtcgtctgc cagctacaac
      121 cgagaaggcg acgtgaaagg aacaagaaag gaggaggagg gcggccgctt acaccacttt
      181 gtacaagaaa gttggcaaca ttttaagaga agacgacgcc ttccgggcgc acgccgagca
      241 gaactccacc gacaccttat ccttgtccac atggagacag actcctcccg ccgagtcgtc
      301 ctcttccagc aggtcctgct tctgctttcc caccggcaga gcgtagtcgt ggtcaccggc
      361 gggcgagtct ctgaagagcg aggtggtcag ccgcagtccc acgccgctca gccggctcag
      421 caagcgagcc agtccagtct cgttggctaa gactgcccgt aggtagcgac tctcctcctg
      481 cagtgcctgt acgcgtttgc ccagctcccg attctcggcc cgcagctcct ggttctcggc
      541 tgccagaccc cggactcgac tctccagccc catcacgtac tccttcttct tcagtcgatt
      601 aaggcgggca gcggccgccg ccgccttccg gggactcttt gtcgccgcct ggttgttgtc
      661 gttaccgctg ccgccaccgc cgcctcctcc tggggacttt ctccgcctct tttcggcgct
      721 gccactgtca ctgctgctgc tgcagcctcc gataccgttt aacagccttt gcagcaggtc
      781 agagaagcgc tgcatttcag cagccgcggc ctcatcgtca tcgtcccctc tccacaggcc
      841 gccgctatcc gagcctccgc cagacgagga gagaggcccc ggcgagctaa gcccggggtc
      901 caggtgccag tccggttgcc tggggtccag gagatccgcc agttccagcc cagacagaaa
      961 gtccatatcc tccgtctctt cccccgggag gctggcgatc gcctcctc
//
