LOCUS       Exported                 980 bp ds-DNA     linear   SYN 07-JUN-2021
DEFINITION  Targets HA-eGFP to mitochondrial intermembrane space (IMS).
ACCESSION   .
VERSION     .
KEYWORDS    pCMV4-SMAC-HA-eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 980)
  TITLE     Unpublished Kahn lab ARF plasmids
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(22..48)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      224..233
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(290..309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(291..309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(354..405)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(383..403)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     619..638
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        713..817
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcgcccttg ctcacgaatt cagcgtaatc tggaacatcg tatgggtagg atccaatagg
       61 aaccgcacac agggttactc caaagccaat cgtcacagtt ttgtgccatg gtcttatcaa
      121 ttctgagaaa caccgcttct taaagttagc cacaacagga acacacaaac actgtctgta
      181 cctgaagaat gaagttacgc tgcgcgacag ccaactcttc agagccgcca tggtacctcc
      241 ggatcgataa ttctagaggg cccgtttaaa cgctagccag cttgggtctc cctatagtga
      301 gtcgtattaa tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg
      361 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      421 ttacgacatt ttggaaagtc ccgttgatac gcgtatatct ggcccgtaca tcgcgaagca
      481 gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg
      541 ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca
      601 gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat
      661 cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac
      721 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      781 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      841 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      901 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      961 tctcaacagc ggtaagatcc
//
