LOCUS Exported 869 bp ds-DNA linear SYN 02-JUN-2021 DEFINITION Standard for cell-free expression benchmarking.. ACCESSION . VERSION . KEYWORDS pCellFree_G03 IL2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 869) AUTHORS Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K TITLE Performance benchmarking of four cell-free protein expression systems. JOURNAL Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. PUBMED 26301602 REFERENCE 2 (bases 1 to 869) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 2, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..869 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 16..39 /codon_start=1 /product="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" /label=HRV 3C site /translation="LEVLFQGP" protein_bind 40..64 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" sig_peptide 64..123 /product="human interleukin-2 signal sequence" /label=IL-2 signal sequence protein_bind complement(524..548) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" terminator 772..818 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" ORIGIN 1 gacgagctat acaagctgga ggtgctcttc cagggtccga caagtttgta caaaaaagtt 61 ggcatgtaca ggatgcaact cctgtcttgc attgcactaa gtcttgcact tgtcacaaac 121 agtgcaccta cttcaagttc tacaaagaaa acacagctac aactggagca tttactgctg 181 gatttacaga tgattttgaa tggaattaat aattacaaga atcccaaact caccaggatg 241 ctcacattta agttttacat gcccaagaag gccacagaac tgaaacatct tcagtgtcta 301 gaagaagaac tcaaacctct ggaggaagtg ctaaatttag ctcaaagcaa aaactttcac 361 ttaagaccca gggacttaat cagcaatatc aacgtaatag ttctggaact aaagggatct 421 gaaacaacat tcatgtgtga atatgctgat gagacagcaa ccattgtaga atttctgaac 481 agatggatta ccttttgtca aagcatcatc tcaacactga cttgcccaac tttcttgtac 541 aaagtggtgt aagcggccgc cctcctcctc ctttcttgtt cctttcacgt cgccttctcg 601 gttgtagctg gcagacgacg agtcttactt ttacgtgtac ttctctatag atgatgtatg 661 atctctctgc atgcgtgttc gtgcatgtgt ccgtgtgttg ggtacgcgtg gtaccctgca 721 ggaaggaagc tgagttggct gctgccaccg ctgagcaata actagtaatt actagcataa 781 ccccttgggg cctctaaacg ggtcttgagg gggttttttg ctgaaaggag gacagctgat 841 gattgtcatg cttgccatct gttttcttg //