LOCUS       Exported                 953 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    GPR61-Tango
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             287..298
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg gaatcaagtc ccataccgca gtccagtggg aactccagta
      301 cgttgggacg cgtgccacag actcccggac catccacagc tagcggcgta cctgaagtgg
      361 gcctccggga cgtggcctcc gaatccgtcg ctttgttctt catgttgctt ctcgacttga
      421 cagccgttgc cggcaacgcc gccgttatgg ccgttatcgc taagacaccg gcactgcgga
      481 aatttgtctt tgtgtttcat ctgtgcctgg tggatttgct cgccgcactg acactcatgc
      541 ctctggccat gctcagcagc tcagcgcttt ttgaccacgc cctcttcggc gaggttgcat
      601 gccggcttta cttgttcctt tccgtttgct ttgtgtccct ggccatactg tctgtgtcag
      661 caataaacgt ggagcgctat tattatgttg tacaccccat gcggtatgaa gtcagaatga
      721 ccctgggcct ggtcgcttcc gtattggtcg gggtttgggt gaaggcactg gccatggcct
      781 cagtgcctgt cctcggaaga gtgtcctggg aagaaggagc cccctccgtg cccccgggct
      841 gctcattgca atggagccac tcagcttatt gccagctgtt cgtggtcgtg ttcgccgtcc
      901 tttactttct gctgcccctc ctgctgatcc tggtcgtgta ctgttcaatg ttc
//
