LOCUS Exported 979 bp ds-DNA linear SYN 14-JUN-2021 DEFINITION Standard for cell-free expression benchmarking.. ACCESSION . VERSION . KEYWORDS pCellFree_G03 IFNG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 979) AUTHORS Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K TITLE Performance benchmarking of four cell-free protein expression systems. JOURNAL Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. PUBMED 26301602 REFERENCE 2 (bases 1 to 979) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 14, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..979 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 164..188 /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(687..711) /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS complement(712..735) /codon_start=1 /product="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" /label=HRV 3C site /translation="LEVLFQGP" primer_bind complement(778..799) /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 ccacgcgtac ccaacacacg gacacatgca cgaacacgca tgcagagaga tcatacatca 61 tctatagaga agtacacgta aaagtaagac tcgtcgtctg ccagctacaa ccgagaaggc 121 gacgtgaaag gaacaagaaa ggaggaggag ggcggccgct tacaccactt tgtacaagaa 181 agttgggtac tgggatgctc ttcgacctcg aaacagcatc tgactccttt ttcgcttccc 241 tgttttagct gctggcgaca gttcagccat cacttggatg agttcatgta ttgctttgcg 301 ttggacattc aagtcagtta ccgaataatt agtcagcttt tcgaagtcat ctcgtttctt 361 tttgttgcta ttgaaaaact tgacattcat gtcttccttg atggtctcca cactcttttg 421 gatgctctgg tcatctttaa agtttttaaa aagtttgaag taaaaggaga caatttggct 481 ctgcattatt tttctgtcac tctcctcttt ccaattcttc aaaatgccta agaaaagagt 541 tccattatcc gctacatctg aatgacctgc attaaaatat ttcttaaggt tttctgcttc 601 ttttacatat gggtcctggc agtaacagcc aagagaaccc aaaacgatgc agagctgaaa 661 agccaagata taacttgtat atttcatgcc aacttttttg tacaaacttg tcggaccctg 721 gaagagcacc tccagcttgt atagctcgtc catgccgaga gtgatcccgg cggcggtcac 781 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg 841 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg 901 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt 961 caccttgatg ccgttcttc //