LOCUS       Exported                 874 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Constitutive expression of a firefly luciferase-enhanced green 
            fluorescent protein-cre recombinase fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    pF CAG luc-EGFP-cre puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Stringer BW, Day BW, D'Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC, 
            Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T, 
            Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis
            PL, Jeffree RL, Johns TG, Boyd AW
  TITLE     A reference collection of patient-derived cell line and xenograft 
            models of proneural, classical and mesenchymal glioblastoma.
  JOURNAL   Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
  PUBMED    30894629
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(231..251)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        236..565
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      416..551
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     478..497
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(575..594)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga
       61 gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac
      121 tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat
      181 taagctagcg ttaactctag agatccgcgg ccgcatcgat tcgaccctgt ggaatgtgtg
      241 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      301 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      361 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
      421 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
      481 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
      541 ttttggaggc ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg
      601 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
      661 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
      721 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
      781 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
      841 cgagatcggc ccgcgcatgg ccgagttgag cggt
//
