LOCUS Exported 12807 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Constitutive expression of firefly luciferase in mammalian cells. ACCESSION . VERSION . KEYWORDS pF CAG luc hygro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12807) AUTHORS Stringer BW, Day BW, D'Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC, Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T, Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis PL, Jeffree RL, Johns TG, Boyd AW TITLE A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. JOURNAL Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z. PUBMED 30894629 REFERENCE 2 (bases 1 to 12807) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12807 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 501..622 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(538..557) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 592..611 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(671..687) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(671..687) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(684..706) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 695..711 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(719..749) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 764..785 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(902..919) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1073..1661) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1153..1172) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1821..2681) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2444..2463 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" primer_bind complement(2857..2876) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 3048..3427 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3429..3627 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 3582..3602 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 3645..3825 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 3872..3997 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 4490..4723 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 4908..4952 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 5250..5367 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 5475..5778 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5781..6056 /label=chicken beta-actin promoter intron 6057..7065 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 6989..7011 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 7073..7092 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" regulatory 7123..7132 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7129..8781 /codon_start=1 /gene="luc" /product="firefly luciferase" /label=luciferase /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" primer_bind complement(7195..7213) /label=LucNrev /note="Firefly luciferase, reverse primer" promoter 8792..9291 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(8827..8850) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 9224..9243 /label=mPGK-F /note="Mouse PGK promoter, forward primer" misc_feature 10325..10913 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(10378..10398) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(10796..10807) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(10916..10932) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10917..10933) /label=pBluescriptKS /note="For pBluescript vector" LTR 11437..11617 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(11643..11660) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 11649..11873 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 11919..12347 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(12006..12025) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 12216..12237 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(12356..12376) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 12361..12690 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 12541..12676 /label=SV40 ori /note="SV40 origin of replication" primer_bind 12603..12622 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 12738..12785 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 12804..371 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" ORIGIN 1 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt 61 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg 121 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca 181 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg 241 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt 301 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg 361 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg 421 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 481 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 541 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 601 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 661 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 721 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 781 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 1021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 1081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 1141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 1201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 1261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 1321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 1381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 1441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 1501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 1561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 1621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 1681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 1741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 1801 aaagtatata tgagtaaact ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 1861 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 1921 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 1981 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 2041 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 2101 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 2161 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 2221 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 2281 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 2341 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 2401 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 2461 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 2521 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 2581 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 2641 aaaaagggaa taagggcgac acggaaatgt tgaatactca tctcttcttt ttatattatt 2701 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 2761 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 2821 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata 2881 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa 2941 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt 3001 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt 3061 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 3121 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 3181 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 3241 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 3301 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 3361 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 3421 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg 3481 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 3541 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg 3601 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag 3661 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 3721 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 3781 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag 3841 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga 3901 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 3961 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 4021 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 4081 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 4141 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 4201 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 4261 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 4321 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 4381 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 4441 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 4501 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg 4561 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 4621 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 4681 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 4741 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 4801 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 4861 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 4921 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 4981 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 5041 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 5101 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 5161 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc 5221 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac 5281 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa 5341 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt 5401 tggttaatta agactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 5461 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 5521 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 5581 ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc agtacatcaa 5641 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 5701 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 5761 gtcatcgcta ttaccatggg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 5821 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 5881 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg 5941 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt 6001 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag 6061 tcgctgcgtt gccttcgccc cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc 6121 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct 6181 gtaattagcg cttggtttaa tgacggctcg tttcttttct gtggctgcgt gaaagcctta 6241 aagggctccg ggagggccct ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt 6301 gtgtgcgtgg ggagcgccgc gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc 6361 gcggcgcggg gctttgtgcg ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc 6421 cccgcggtgc gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg 6481 gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac ccccccctgc acccccctcc 6541 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg 6601 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc 6661 tcgggccggg gagggctcgg gggaggggcg cggcggcccc ggagcgccgg cggctgtcga 6721 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt 6781 cctttgtccc aaatctggcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg 6841 ggcgcgggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc 6901 gtcgccgcgc cgccgtcccc ttctccatct ccagcctcgg ggctgccgca gggggacggc 6961 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct 7021 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg 7081 ctggttattg tgctgtctca tcattttggc aaaggatccg ccgccaccat ggaagacgcc 7141 aaaaacataa agaaaggccc ggcgccattc tatcctctag aggatggaac cgctggagag 7201 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat 7261 gcacatatcg aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca 7321 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac 7381 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc 7441 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgaacatttc gcagcctacc 7501 gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaaattacca 7561 ataatccaga aaattattat catggattct aaaacggatt accagggatt tcagtcgatg 7621 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtaccagag 7681 tcctttgatc gtgacaaaac aattgcactg ataatgaatt cctctggatc tactgggtta 7741 cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat 7801 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat 7861 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta 7921 atgtatagat ttgaagaaga gctgttttta cgatcccttc aggattacaa aattcaaagt 7981 gcgttgctag taccaaccct attttcattc ttcgccaaaa gcactctgat tgacaaatac 8041 gatttatcta atttacacga aattgcttct gggggcgcac ctctttcgaa agaagtcggg 8101 gaagcggttg caaaacgctt ccatcttcca gggatacgac aaggatatgg gctcactgag 8161 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa 8221 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt 8281 aatcagagag gcgaattatg tgtcagagga cctatgatta tgtccggtta tgtaaacaat 8341 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct 8401 tactgggacg aagacgaaca cttcttcata gttgaccgct tgaagtcttt aattaaatac 8461 aaaggatatc aggtggcccc cgctgaattg gaatcgatat tgttacaaca ccccaacatc 8521 ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt 8581 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt 8641 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa 8701 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag 8761 ggcggaaagt ccaaattgta agctagctac cgggtagggg aggcgctttt cccaaggcag 8821 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg 8881 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc 8941 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg 9001 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc 9061 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc 9121 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg 9181 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa 9241 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcatc 9301 ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa 9361 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca 9421 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct 9481 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc 9541 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg 9601 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg 9661 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac 9721 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc 9781 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc 9841 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg 9901 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga 9961 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt 10021 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag 10081 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct 10141 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc 10201 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga 10261 ccgatggctg tgtagaagta cttcgccgat agggcgcgcc gaattcgata tcaagcttat 10321 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 10381 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 10441 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 10501 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 10561 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 10621 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 10681 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct 10741 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 10801 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 10861 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata 10921 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac 10981 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac 11041 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 11101 aaaagaaaag gggggactgg agggctaatt cactcccaac gaagacaaga tatccttgat 11161 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca 11221 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag 11281 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat 11341 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt 11401 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc 11461 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 11521 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 11581 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc 11641 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 11701 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 11761 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 11821 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 11881 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 11941 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 12001 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 12061 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 12121 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 12181 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 12241 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 12301 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 12361 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 12421 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 12481 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 12541 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 12601 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 12661 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 12721 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat 12781 acgacaaggt gaggaactaa accatgg //