LOCUS       Exported                 912 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    NMBR-Tango
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg ccctccaagt ctctgtcaaa tctgagtgtc accaccggag
      301 ctaatgagag tggatctgtt cctgagggat gggaaaggga tttccttcca gctagcgatg
      361 ggaccacaac agagcttgtg atccggtgcg tgatcccctc actctacttg ctgatcatta
      421 cagtcgggct tctcggcaac ataatgctcg ttaagatttt cataactaat tcagccatgc
      481 ggtccgttcc caacatcttt atcagtaacc tcgctgcagg tgatctgctc ctgcttctga
      541 cctgcgtacc tgtggacgca tcccgctact tttttgatga atggatgttt ggaaaggttg
      601 ggtgtaagct gatccctgtc atccagctca cgtcagtggg ggtgagtgtg ttcacgctta
      661 ctgccctctc tgcagataga taccgagcga tcgtaaaccc catggatatg cagacgtccg
      721 gcgctcttct ccggacttgc gtgaaggcaa tgggaatatg ggtggtgtca gtgttgctgg
      781 ctgttcccga ggccgtgttt tcagaggttg ctaggatctc ttcactggat aatagttcat
      841 tcacggcctg tattccatat ccccagacag acgagctgca ccctaaaatc cactccgtac
      901 ttatattttt gg
//
