LOCUS       Exported               14546 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Cas9 driven by double 35S, hygromycin resistance for plant 
            selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    p201H Cas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14546)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA.
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnology. 2015;15:16
REFERENCE   2  (bases 1 to 14546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14546
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2250..3317
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3383..3577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3813..3835)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3921..4061
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3976..3995
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4076..4093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4247..4835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4327..4346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4930..5745)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5653..5672
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5737..5756
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    6038..6062
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6239..6260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6275..6305
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6313..6329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6318..6340
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6337..6353
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6337..6353
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(6807..7832)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        8978..9654
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9591..9611
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9700..13803
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13816..13836
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      13903..14155
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(14197..14214)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14197..14213)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14206..14228)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    14409..14433
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg caggatcatg
     4921 aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     4981 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     5041 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     5101 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     5161 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
     5221 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     5281 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
     5341 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     5401 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
     5461 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     5521 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     5581 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     5641 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     5701 taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     5761 actgtttatg taagcagaca gttttattgt tcatgatctg gatcacaggc agcaacgctc
     5821 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     5881 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     5941 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     6001 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6061 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     6121 acgccgaatt gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6181 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6241 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6301 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     6361 cgaattcctt gactagaggg taggcgcggc ttaattgctt aaatttacta tttttttact
     6421 atattatact caacccaatg agcataaaga ctgtaaaatc tcaaattcct gagaagcata
     6481 tttatcgatc ccacagactt gatagttcca taatccatac gctgcagcca aattgctagt
     6541 gtgttgaaca tttaacacgt agagaactag aaaagatata aaactaagat tgatatccaa
     6601 aatagacgag aacaataagc aaaaactctt agttttgaaa taaatcaaca atcccgaggg
     6661 ttgtcacata catcaaaaac gaaaatccat atagcaaaaa aaactctaaa ttaccgttcg
     6721 acaaaaagag aaaactgata ggacatttgc taaacattaa aatcaaaatt tggatcaagc
     6781 cgaattgatc ccggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt
     6841 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     6901 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     6961 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     7021 agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc
     7081 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     7141 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     7201 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     7261 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     7321 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     7381 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     7441 ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga
     7501 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag
     7561 atgcaatagg tcaggctctc gctgaatgcc ccaatgtcaa gcacttccgg aatcgggagc
     7621 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     7681 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     7741 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg
     7801 acagacgtcg cggtgagttc aggctttttc atctcgagac gtaccgcgcg atgcattcga
     7861 agatcgatcc ttcgcctgga ggagagaaat cagtggcgct gcggctttta gggtttcttt
     7921 gttgatggaa tgagagtgta agctctgcca gtgccacttt attagggttt tacaagccct
     7981 tttcttcgta attgggcctg acattttgtg ccacttgggc ctttagagat gaaaatgtat
     8041 attgggctta agttgacttg aaggataaat tagtttagga tattacgttt tttatgagaa
     8101 ttggtgtgtc ggatacatgt atatgatgca ttcaaatata tgtattctag atacatttaa
     8161 gtttagatac aatctaaaat gtgtctttaa ttacaggact gtaactaaaa tacttaatgt
     8221 aagaagaata ttactccttt aatagctttt gagtatatct agtctaacat cttttaaaaa
     8281 agtctaattt ctttcattta tttttcgagc aatagcaaag tgcataatta tttttttctt
     8341 ctagaaattc agatttgttt ctctaaattt tgagattctt ttctcaattt tgtatgtcta
     8401 gagaacaatg tgtatttttc actctagttg gttgttactt tgttgaatgt tctgataaaa
     8461 gtatattgtt atttctgaag tagatataaa ccttcatttg gaaattatac ataaatcaaa
     8521 atcgttaatt atctagatca agatatatgc ccttttccta atgtatttga tacatgcacc
     8581 taatttcact agatgtatct tttctatttt ttaaattatg aatagttaat tttttccata
     8641 tgtgtatttg atacatactt catgacttta aaaaattaat tatataccag atatatgtat
     8701 ttaaaatttg ttatgtattt aaagtatgta tatgattatt cgatattaat ctcttcgatg
     8761 aaatttaaat cgataactat gtgctttgga tcgatctgcc cactagtgag tcgtattaca
     8821 attcatgact ctcttaaggt agccaaaatc gatatctagg gataacaggg taatcgatca
     8881 ctagaattcg gcgcgccggg cccaacatgg tggagcacga cactctcgtc tactccaaga
     8941 atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
     9001 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag
     9061 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
     9121 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     9181 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgaa catggtggag
     9241 cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct
     9301 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct
     9361 atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat
     9421 tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga
     9481 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     9541 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     9601 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctgaaatcac
     9661 cagtctctct ctacaaatct atctctctcg accgctagca tggacaagaa gtactccatt
     9721 gggctcgata tcggcacaaa cagcgtcggc tgggccgtca ttacggacga gtacaaggtg
     9781 ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa gaagaacctc
     9841 attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct caaaagaaca
     9901 gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga gatctttagt
     9961 aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc ctttttggtg
    10021 gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga cgaggtggcg
    10081 taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga cagtactgat
    10141 aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt tcggggacac
    10201 ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact ctttatccaa
    10261 ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc cggagttgac
    10321 gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca
    10381 cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct gtcactcggg
    10441 ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct tcaactgagc
    10501 aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga ccagtacgca
    10561 gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga tattctgcga
    10621 gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg ctatgatgag
    10681 caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc tgagaagtac
    10741 aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga cggcggagca
    10801 agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga cggcaccgag
    10861 gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac tttcgacaat
    10921 ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag gcggcaagag
    10981 gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct cacatttcgg
    11041 ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg gatgactcgc
    11101 aaatcagaag agaccatcac tccctggaac ttcgaggaag tcgtggataa gggggcctct
    11161 gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa cgaaaaggtg
    11221 cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct caccaaggtc
    11281 aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca gaagaaagct
    11341 atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct caaagaagac
    11401 tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga ggatcgcttc
    11461 aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa ggacttcctg
    11521 gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac gttgtttgaa
    11581 gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga cgacaaagtc
    11641 atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag aaaactgatc
    11701 aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa gtccgatgga
    11761 tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt taaggaggac
    11821 atccagaaag cacaagtttc tggccagggg gacagtcttc acgagcacat cgctaatctt
    11881 gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt ggatgaactc
    11941 gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc ccgagagaac
    12001 caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat tgaagagggt
    12061 ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac ccagcttcag
    12121 aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt ggatcaggaa
    12181 ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca gtcttttctc
    12241 aaagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag agggaagagt
    12301 gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg gcagctgctg
    12361 aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga acgaggtggc
    12421 ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac acgccagatc
    12481 accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga tgaaaatgac
    12541 aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtctc agatttcaga
    12601 aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc gcatgatgcc
    12661 tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct tgaatctgaa
    12721 tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa gtctgagcag
    12781 gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa ttttttcaag
    12841 accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga aacaaacgga
    12901 gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg gaaggtcctg
    12961 tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg cttctccaag
    13021 gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa agattgggac
    13081 cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact ggttgtggcc
    13141 aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct gggcatcaca
    13201 atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc gaaaggatat
    13261 aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt tgagcttgaa
    13321 aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa cgagctggca
    13381 ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa gctcaaaggg
    13441 tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca ctaccttgat
    13501 gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga cgctaacctc
    13561 gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga gcaggcagaa
    13621 aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt caagtacttc
    13681 gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga cgccacactg
    13741 attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca gctcggtgga
    13801 gacagcaggg ctgaccccaa gaagaagagg aaggtgtgac cgcggatatc tgcagaagct
    13861 tacgcgtcga cgtctcgagg ccggccgagc tcgaatttcc ccgatcgttc aaacatttgg
    13921 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
    13981 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
    14041 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
    14101 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
    14161 ttgatatcgt agggataaca gggtaataag cttggcactg gccgtcgttt tacaacgtcg
    14221 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
    14281 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
    14341 gaatggcgaa tgagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
    14401 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
    14461 aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
    14521 aaccacaggg ttcccctcgg gatcaa
//
