LOCUS Exported 10799 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone. ACCESSION . VERSION . KEYWORDS pFUGW-hGtACR1-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10799) AUTHORS Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL TITLE Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics. JOURNAL Science. 2015 Jun 25. pii: aaa7484. PUBMED 26113638 REFERENCE 2 (bases 1 to 10799) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10799 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2611..3821 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3658..3677 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" CDS 4750..5466 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(4792..4813) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5053..5072) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5400..5421 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5491..6079 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5544..5564) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5962..5973) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6082..6098) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6083..6099) /label=pBluescriptKS /note="For pBluescript vector" LTR 6604..6784 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6810..6827) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6816..7040 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7086..7514 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7173..7192) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7383..7404 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7523..7543) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7528..7857 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7708..7843 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7770..7789 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7905..7952 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7971..8345 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8475..8596 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8512..8531) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8566..8585 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8645..8661) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8645..8661) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8658..8680) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8669..8685 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8693..8723) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8738..8759 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8876..8893) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9047..9635) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9127..9146) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9806..10666) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10429..10448 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10667..10771) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg 2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc 2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag 2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg 2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg 2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac 2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc 3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct 3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag 3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga 3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg 3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga 3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc 3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt 3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg 3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg 3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg 3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga 3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg 3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat 3781 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc 3841 gactctagag gatccatgag cagcatcacc tgtgatcccg ccatctacgg cgaatggagc 3901 agggagaacc agttctgcgt ggagaagagc ctgatcaccc tggacggcat caagtacgtc 3961 cagctggtga tggccgtcgt gagcgcctgt caggtgttct tcatggtgac cagagccccc 4021 aaggtgccct gggaagccat ctacctgccc accaccgaga tgatcaccta ttccctggcc 4081 ttcaccggaa acggctacat cagagtggcc aatggcaagt acctgccctg ggccagaatg 4141 gccagctggc tgtgcacctg ccctatcatg ctgggcctgg tgagcaatat ggccctcgtg 4201 aagtacaaga gcatccccct caaccctatg atgatcgccg cctccagcat ctgcaccgtg 4261 ttcggcatca ccgcctccgt ggtgctagac ccgctgcacg tgtggctgta ctgtttcatc 4321 agcagcatct tcttcatctt cgagatggtg gtggccttcg ccattttcgc cattaccatc 4381 cacgatttcc agaccatcgg ctcccccatg tccctgaagg tggtggagag gctgaagctg 4441 atgaggatcg tgttctacgt gagctggatg gcctacccta tcctgtggag cttctccagc 4501 accggcgcct gcatcatgag cgagaacacc agcagcgtgc tgtacctgct gggcgacgct 4561 ctgtgcaaga acacctacgg catcctgctg tgggctacaa cctggggcct gctgaacggc 4621 aagtgggaca gggattacgt gaagggcagg aacgtggacg gcaccctgat gcctgagtac 4681 gagcaggacc tggagaaggg caacaccgag aggtacgagg acgccagagc cggcgagacc 4741 gcggccgccg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 4801 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 4861 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 4921 cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac 4981 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 5041 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 5101 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 5161 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 5221 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 5281 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 5341 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 5401 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 5461 aagtaagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa 5521 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5581 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5641 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 5701 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 5761 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5821 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 5881 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 5941 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6001 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 6061 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca 6121 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag 6181 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag 6241 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 6301 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct 6361 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc 6421 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc 6481 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag 6541 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt 6601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 6661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 6721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 6781 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 6841 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 6901 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 6961 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 7021 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 7081 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 7141 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 7201 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 7261 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 7321 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 7381 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 7441 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 7501 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 7561 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 7621 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 7681 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 7741 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 7801 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 7861 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat 7921 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt 7981 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga 8041 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg 8101 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg 8161 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca 8221 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc 8281 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg 8341 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 8401 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 8461 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 8521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 8581 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 8641 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 8701 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 8761 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 8821 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 8881 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 8941 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 9001 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 9061 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 9121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 9181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 9241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 9301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 9361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 9421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 9481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 9541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 9601 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 9661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 9721 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 9781 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 9841 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 9901 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 9961 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 10021 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 10081 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 10141 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 10201 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 10261 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 10321 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 10381 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 10441 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 10501 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 10561 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 10621 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 10681 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 10741 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac //