LOCUS Exported 932 bp ds-DNA linear SYN 14-JUN-2021 DEFINITION Standard for cell-free expression benchmarking.. ACCESSION . VERSION . KEYWORDS pCellFree_G03 WWP1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 932) AUTHORS Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K TITLE Performance benchmarking of four cell-free protein expression systems. JOURNAL Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. PUBMED 26301602 REFERENCE 2 (bases 1 to 932) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 14, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..932 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(14..37) /codon_start=1 /product="8xHis affinity tag" /label=8xHis /translation="HHHHHHHH" primer_bind complement(180..199) /label=T7 /note="T7 promoter, forward primer" promoter complement(181..199) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 231..335 /gene="bla" /label=AmpR promoter CDS 520..898 /label=ISS /note="immunostimulatory sequence from the AmpR gene; contains unmethylated CpG dinucleotides in the context of 5'-AACGTT-3' (Sato et al., 1996)" primer_bind complement(554..573) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 ccttgcttgt gccgtggtga tggtggtgat ggtggtgacc agaggtgccc atggtttcac 61 ttacgtgttt aatgtcttta cagactttat acattactgt catggttaaa taaaataaaa 121 taaaataaaa taaaataaaa taaaataaaa taaaataaaa taaaataaac ttaagatgtc 181 cctatagtga gtcgtattag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 241 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 301 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 361 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 421 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 481 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 541 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 601 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 661 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 721 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 781 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 841 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 901 gcaaactatt aactggcgaa ctacttactc ta //