LOCUS Exported 950 bp ds-DNA linear SYN 14-JUN-2021 DEFINITION Standard for cell-free expression benchmarking.. ACCESSION . VERSION . KEYWORDS pCellFree_G03 WWP1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 950) AUTHORS Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K TITLE Performance benchmarking of four cell-free protein expression systems. JOURNAL Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. PUBMED 26301602 REFERENCE 2 (bases 1 to 950) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 14, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..950 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 118..141 /codon_start=1 /product="8xHis affinity tag" /label=8xHis /translation="HHHHHHHH" CDS 148..858 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITLGMDELYK" primer_bind complement(187..208) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(448..467) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 795..816 /label=EGFP-C /note="EGFP, forward primer" CDS 859..882 /codon_start=1 /product="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" /label=HRV 3C site /translation="LEVLFQGP" protein_bind 883..907 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 tttattttat tttattttat tttattttat tttattttat tttatttaac catgacagta 61 atgtataaag tctgtaaaga cattaaacac gtaagtgaaa ccatgggcac ctctggtcac 121 caccatcacc accatcacca cggcacaagc aagggcgagg agctgttcac cggggtggtg 181 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 241 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 301 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 361 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 421 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 481 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 541 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 601 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 661 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 721 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 781 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 841 atggacgagc tatacaagct ggaggtgctc ttccagggtc cgacaagttt gtacaaaaaa 901 gttggcatgg ccactgcttc accaaggtct gatactagta ataaccacag //