LOCUS Exported 9906 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses full-length P. vivax MSP3.4 (PVX_097685) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence. ACCESSION . VERSION . KEYWORDS MSP3.4-bio SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9906) AUTHORS Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, Rayner JC TITLE A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals New Vaccine Candidates and Protein-Protein Interactions. JOURNAL PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 10.1371/journal.pntd.0004264. eCollection 2015 Dec. PUBMED 26701602 REFERENCE 2 (bases 1 to 9906) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9906 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 154..533 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 534..737 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 688..708 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 734..758 /label=LNCX /note="Human CMV promoter, forward primer" primer_bind 1239..1258 /label=pMT2-F /note="Synthetic intron, forward primer" regulatory 1281..1290 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 5286..5341 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(5340..5359) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(7577..7595) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7663..7767 /gene="bla" /label=AmpR promoter CDS 7768..8628 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7986..8005) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8799..9387 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9288..9307 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9541..9558 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9675..9696 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9711..9741 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9749..9765 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9754..9776 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9773..9789 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9773..9789 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat 61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt 121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat 181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc 421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac 481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg 721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg 781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt 841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc 901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg 961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg 1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg 1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat 1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc 1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt 1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc 1321 tgctctgcat gtctggtgcg gccgccaatg tggtgtccaa cgagatcgtg aacctgaaga 1381 accccaacct gcggaacggc tgggccggca aggatgtgac actgcaggac gagcagaacg 1441 gcctgaacgc cgaggatggc gacgagacag atcccaacgg cgcccacaat gagctgctgc 1501 agaagtccgg aaaggccgag ctgcagatgc aggcccagga cagcgacagc aaagaggaca 1561 tcaagaccaa gatcgacgag ctcgtgaaca aggccgaggc cgccaacacc aaggccgata 1621 cagccgtgac agaggccgag aagctgctga aaaccgccac cgaggccgaa aagcagatca 1681 aggtggacga ggaaaaagag acaaccgccg ccacaaaggc caaagagcag gctgagaagg 1741 cctttgccga ggccaagatc aagaaagagg aagccaagac aaaaaaagag gacgctaagg 1801 ccaaggctaa ggccgacgac gccaagaaga agtccgagga aaccgacgaa gccgagctga 1861 aggccaagct ggaagaggtg gaaaagctcg tgggccaggc cgaacaggcc gcctctgatg 1921 ccgagaatga gcagaaaaag gccgaatggg ccgccaagat tgccgaggaa ctgaagaagg 1981 cctacgaggc caaaaaaaag gcccgcgaga gcaaggtggc cgccgagaac gccaaaaacg 2041 atgccacatc tgccgccagc ggcagcaagg tgcagaaagt gaccgaggcc atcaagatcg 2101 ccgacgatga gatgcagacc gccgctaaga atgccgccga agccgaaggc gccgagagaa 2161 aagctgagca ggccgctagc gaggccatta acgccatcga gcagaaggtg gacgcccaga 2221 gcaaagtgaa cgtggtgcag aacgaggccg acgctgcctc taaagccgcc accgctgcca 2281 agaacgccca gaccaaggct gagatcgccg tgttcgtcgt gaaaaccgag gtgtccaaag 2341 aagaggccgt gaagtccgcc aacgccgccg aggaagctaa aacaaaggct gaggaagtgg 2401 ccaccaagag cgaggaaatc aagatcacca acgtggccaa gtccgagaaa gtgaaaagcg 2461 ccaagtctga agccgtgaat caggccgctg ccgccgacaa ggcaaagaaa gaagctgagg 2521 ctgccgccaa ctctgccgat aaggcccaga caagcaccaa gggcatcgac aacgaggcca 2581 ccctgagcac cgccaaaaaa gaagccgaaa cagccgccag caacgccggc aaagcccaga 2641 cagatgcctc taccgccctg gccaaagcca agaaggctca gatcgagaca gagatcgctg 2701 ccgaggtcgt gaaggcagaa aaggctaaga aggacgccag cagcgacgcc gatgccgctg 2761 tggaagccag aaagaaagcc gagagcgagg cagagaaagc caagtccaat gaggtggccc 2821 aggaagccgc ccagaaggct aaagaacagg aagccgaggc cctgaaagcc aagggcaatg 2881 cccagaccgc cagcgaccag gccgaaaaag aagcagaggg cgacggcacc gaggacaagc 2941 tggctaaagt gaaagagaaa gaaaaggacg cctccaatgc cgccacagcc gctaaaaacg 3001 ccagaaccac agccgagatt gccgcagagg tggccaaggc ccaggctgct aagacagagg 3061 cagaggccgc taaagtggcc gctaacgcag ccaaggacga ggctcagaaa gtggctgacg 3121 agtccaagag cgctgaggca cagaaggccg cagagaaggc tagcgaagcc gctacaacag 3181 ccggcgagaa agccgctgaa gctgccaagg tggcagatga cgccaccagc gtgtccaaca 3241 ccgccaccct gtccaccatg caggaaaagg ccaacaaagc cgtggaacac gccaaagaag 3301 caaaaaaggc acagaccaaa gcagagctgg ctgccgaaga agccaaggca gaagcagcta 3361 agaaagaggc ccagaaagct caggaagctg ccgacgccgc taaaaaagag gccgaggaaa 3421 tcgccaagaa agaaaaagtg gatgccaagg ctgtggccga agccacctcc gccctggata 3481 ccgcaaagac taaggcaaaa gaggcaaagg acaaggccgg cgacgtgtca aaagaagtgg 3541 aaaaagctga aaaagaggtg gaaaaagtcg tgggggacga cgcccagaaa agcgagatca 3601 ccacagccgt gtccggcatc aagggcaaga cctctgaggc cgtgcaggcc gcaaaggatg 3661 caaaaaaagc caaagacctg gccgagatcg ccctgaatgt cgtgaaagct gaagtggcta 3721 acgacaaggc taaaaaggct gtgcccgctg ccggcgtggc caaagaggat gccaaaaagg 3781 ctgccgctag cgccgagcag cccaagaaag aggctgagag cgccgaggaa ggcgccgaaa 3841 tcgccaaaga gaacgagatc gaggccggcc agaaagccaa agaagccgag gataacgccg 3901 ccgtggtgca ggccaaactg ctgatcgccg agcaggaact gaaaaacgcc aaggccgaga 3961 acgacgccga gaagttccag agcgccaagg caaatgccct ggccgctgct gaggaagctg 4021 tgaagaaagg ccaggccgca gaagccgctg ccaacgctgc tacagacaaa gccaaaaaag 4081 ctgctgaggc cgccgaaaag gctaaaaaag ccgctgccga gtccgccctg aagaaaaaac 4141 tgaaggtgct ggaaatcgtg aagaagtaca gcaagcgggg ctacaacgtg gtggacaacg 4201 atgaacaggt gctgaacgaa gtggatgagc aggcttccga agctaaagaa gaagaggacg 4261 aggacgaagc agacgacagc gccagctccg acgtggaaat cgaggacgac gaggaagagg 4321 aagaagaaga ggacgaagaa gtgaaagagg aagagggcca ggcccagagc agcgcccatc 4381 agaatagtgt ggctgagctg gaaaaccaga agaaagagag ccaggaaaag tccgacgagc 4441 cccccagcga caacaacgct caggccctgc tgagcgacaa ctacaagaac ttcgccgact 4501 tcaagaagaa agctgaggac ctgaccaaga acatcatcaa caccatcgac ggcgacgctg 4561 gcgtgatcga caccctgaag gacttcgcca acgacgtgaa ccagttcatc ctgaacatgg 4621 gcgcgccgtc gacctccatc acggcctata agagtgaggg ggagtcagcg gagttctcct 4681 tcccactcaa ccttggagag gaaagcctgc agggagagtt gagatggaag gcagagaagg 4741 ctccttcttc ccagtcctgg atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga 4801 agtctactag caaccccaag ttccagctgt ccgaaacgct cccactcacc cttcagatac 4861 cccaggtctc ccttcagttt gctggttctg gcaacctgac cctgactctg gacagaggga 4921 tactgtatca ggaagtgaac ctggtggtga tgaaagtgac tcagcccgac agcaacactt 4981 tgacctgtga ggtgatggga cccacctcac ccaagatgag actgatcttg aagcaggaga 5041 atcaggaggc cagggtctcc aggcaggaga aagtgattca agtgcaggcc cctgaagcag 5101 gggtgtggca atgtctactg agtgaaggtg aagaggtcaa gatggactcc aagatccagg 5161 ttttatccaa agggttgaat tccggatcac tgcatcatat tctggatgca cagaaaatgg 5221 tgtggaatca tcgttaagtc tagacacgtg attaattaag gatcccccga cctcgacctc 5281 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc 5341 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc 5401 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct 5461 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg 5521 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat 5581 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt 5641 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt 5701 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac 5761 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag 5821 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta 5881 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca 5941 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca 6001 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt 6061 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc 6121 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag 6181 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac 6241 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg 6301 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg 6361 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa 6421 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca 6481 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg 6541 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc 6601 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg 6661 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata 6721 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact 6781 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt 6841 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt 6901 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat 6961 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc 7021 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc 7081 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc 7141 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc 7201 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac 7261 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc 7321 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc 7381 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc 7441 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc 7501 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt 7561 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 7621 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 7681 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 7741 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 7801 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 7861 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 7921 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 7981 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc 8041 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 8101 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 8161 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 8221 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 8281 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta 8341 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 8401 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 8461 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 8521 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 8581 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 8641 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 8701 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 8761 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 8821 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 8881 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 8941 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 9001 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 9061 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 9121 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 9181 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 9241 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 9301 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 9361 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 9421 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 9481 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 9541 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 9601 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 9661 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 9721 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 9781 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca 9841 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg 9901 tatgga //