LOCUS       Exported                7917 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses full-length P. vivax MSP7.6 (PVX_082675) ectodomain in 
            mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    MSP7.6-bio
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    3297..3352
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3351..3370)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5588..5606)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5674..5778
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5779..6639
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5997..6016)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6810..7398
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7299..7318
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7552..7569
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7686..7707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7722..7752
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7760..7776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7765..7787
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7784..7800
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7784..7800
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcagctg atgcttctga tggcgtgccc atgctgttca
     1381 acgacgagaa ccatctgccc aacagcctga accggaagct gcggaacctg aacgagatcg
     1441 tgcaggaaga tgtgtccgag aacagccacg aggtggacga gctggaaaag cacgaggaag
     1501 aggaagagga cgtggaagtg gaagaagaac agcacgaaca gcagcacggc cacaccgtgg
     1561 aagatcccag agaaggcgac gagggcaacg agaacgaccc ccaggaaggc acccacgagc
     1621 aggacgacga ggacttcatg ggccagtgga ccgaagtgat ggccaacgag gaaggcgata
     1681 gcggcgaggg cgaggacgat accgacagcg aggatgatgc cgagggggag agcaacaccc
     1741 acagccctag aagccctccc gtggaaggct ctgatgccac agtgctgcag gccgagccta
     1801 gcacagccct gagcgacagc agcaactctg cccagattgg ccagcagggc gtgcacagac
     1861 caccagccgg actggccgat cagaccaccc agagaacagg cgatcagggc gccgatggac
     1921 ctcatggcga ttctggcgcc gctacctcta gagccggcgg atctgctgag agaggcccct
     1981 ctggcgaacg gcctcaggaa agagcacctg gcggaacccc tgagctgccc gccaattctg
     2041 tgcctcagag agagggcaga agcgacgccc tgccacagga acagcaggcc cagtctgaag
     2101 tcgtgcggag cgagagacag tctggcgctg ctgcagatgg cggagcccag ccttctgaaa
     2161 gacctgccgc tcctccacag cagaccagca gagacacacc ccctaagggc accgccgaca
     2221 gcaacgacgc caagatcaag tacctggaca agctgtacga cgaggtgctg accacctccg
     2281 acaacaccgc cggaatccac gtgcccgact accacagcaa gtacaacacc atccggcaga
     2341 aatacgagta cagcatgaac cccgtggaat atgagatcgt gaagaacctg ttcaatgtgg
     2401 gcttcaagaa cgacggcgct gccagcagtg atgccacccc tctggtggac gtgttcaaga
     2461 aggccctggc cgacgagaag ttccaggccg agttcgacaa cttcgtgcac ggcctgtacg
     2521 gcttcgccaa gcggcacagc tacctgagca aagaacggat gggcgacacc acccggtaca
     2581 gcgacctgct gaagaacgcc atctccctga tgaacaccat cgaagtgaat ggcgcgccgt
     2641 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     2701 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     2761 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     2821 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     2881 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     2941 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     3001 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     3061 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     3121 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     3181 aagggttgaa ttccggatca ctgcatcata ttctggatgc acagaaaatg gtgtggaatc
     3241 atcgttaagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     3301 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3361 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     3421 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     3481 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     3541 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     3601 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     3661 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     3721 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     3781 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     3841 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     3901 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     3961 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     4021 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     4081 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     4141 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     4201 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     4261 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     4321 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     4381 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     4441 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     4501 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     4561 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     4621 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     4681 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     4741 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     4801 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     4861 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     4921 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     4981 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     5041 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5101 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5161 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     5221 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     5281 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     5341 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5401 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     5461 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     5521 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     5581 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5641 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5701 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5761 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5821 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5881 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5941 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6001 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     6061 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6121 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6181 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6241 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6301 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     6361 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6421 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6481 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6541 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6601 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6661 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6721 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6781 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6841 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6901 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6961 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7021 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7081 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7141 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7201 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7261 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7321 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7381 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7441 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7501 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7561 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7621 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7681 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7741 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7801 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     7861 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatgga
//
