LOCUS Exported 7725 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses full-length P. vivax MSP7.9 (PVX_082655) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence. ACCESSION . VERSION . KEYWORDS MSP7.9-bio SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7725) AUTHORS Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, Rayner JC TITLE A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals New Vaccine Candidates and Protein-Protein Interactions. JOURNAL PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 10.1371/journal.pntd.0004264. eCollection 2015 Dec. PUBMED 26701602 REFERENCE 2 (bases 1 to 7725) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7725 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 154..533 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 534..737 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 688..708 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 734..758 /label=LNCX /note="Human CMV promoter, forward primer" primer_bind 1239..1258 /label=pMT2-F /note="Synthetic intron, forward primer" regulatory 1281..1290 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 3105..3160 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(3159..3178) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(5396..5414) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5482..5586 /gene="bla" /label=AmpR promoter CDS 5587..6447 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5805..5824) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6618..7206 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7107..7126 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7360..7377 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7494..7515 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7530..7560 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7568..7584 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7573..7595 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7592..7608 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7592..7608 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat 61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt 121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat 181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc 421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac 481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg 721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg 781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt 841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc 901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg 961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg 1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg 1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat 1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc 1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt 1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc 1321 tgctctgcat gtctggtgcg gccgcagaga agctgggcat ccagaagaag aaaaagaacc 1381 tggaacagga cgccatgcac gccctgatga agaagctgga aagcctgtac aagctgagcg 1441 ccaccgacaa cagcgagatc ttcaacaaag agatggaagc cctgaagaag cagatcgacc 1501 agctgcagca gcacggcgga gccaacgagg aagagtctct gggccatctg ctggaatccg 1561 aggccgccga cgacagcagc aagaaaacca tcttcggcgt ggacgaggac gacctggaca 1621 actacgacgg cgacttcacc ggccagagca agggcaagat caagggccag gccgataccg 1681 cccctagcgt gaagggggat gtgtcccccc ctcctaatct gcctgccgcc gctgccagca 1741 gccccaaaga aacagtgcct gccggcacca gcaacggcct ggtggaagcc gattacgtgg 1801 tgctgaacac ccccgacggc aaccctagac ctgtgggacc tggcggcgga agcagacctt 1861 ctgcctctgg acctgacgcc gccagcaacc tgcagaatca ggccacagct gaagccggcg 1921 gatccaccaa tacccagggc tctcagaccg gcggagtgtc tacaacccct ggcgccaatc 1981 agcaggccgg ctctactcct cctggcgccg ctacagccca gacagagtct gagagggaac 2041 ccaacgtgaa gtacctggat aagctgtacg acgacgtgct ggccacccag gacagcgcca 2101 atggcatcga cgtgccccca ttccacagca agtacaacga ctttcggaag aaatacgagt 2161 tcaagatgaa cgacgccgag tacaagatca tgaagaacct gttcgacgtg tccttcaaga 2221 aagagggcca gcagagcagc gccgcctgcc tcgtgaacgt gttcaagaag gtgctggacg 2281 acgagcatct gcagaaagaa ttcgacaact tcgtgcacgg cttctacggc ttcgccaagc 2341 ggcacaacta cctgcgggga gagagaatgg ccaacgagaa tctgtacaag gacatcttca 2401 agaacgtcgt gaacctgctg aataccatcg aggtcgtggg cgcgccgtcg acctccatca 2461 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg 2521 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga 2581 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt 2641 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg 2701 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc 2761 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac 2821 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca 2881 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga 2941 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt 3001 ccggatcact gcatcatatt ctggatgcac agaaaatggt gtggaatcat cgttaagtct 3061 agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat 3121 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag 3181 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc 3241 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat 3301 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg 3361 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca 3421 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca 3481 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg 3541 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct 3601 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc 3661 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact 3721 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta 3781 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt 3841 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga 3901 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg 3961 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa 4021 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt 4081 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa 4141 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga 4201 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat 4261 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt 4321 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt 4381 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag 4441 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg 4501 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg 4561 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc 4621 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat 4681 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc 4741 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat 4801 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt 4861 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt 4921 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt 4981 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt 5041 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca 5101 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca 5161 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta 5221 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca 5281 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca 5341 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc 5401 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt 5461 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 5521 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 5581 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 5641 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 5701 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 5761 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 5821 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 5881 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 5941 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 6001 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 6061 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 6121 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 6181 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 6241 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 6301 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 6361 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 6421 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 6481 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 6541 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 6601 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 6661 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 6721 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 6781 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 6841 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 6901 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 6961 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 7021 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 7081 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 7141 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 7201 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 7261 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 7321 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 7381 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 7441 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 7501 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 7561 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 7621 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg 7681 caacgttgtt gccattgctg caggcgcaga actggtaggt atgga //