LOCUS Exported 7938 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses full-length P. vivax MSP10 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence. ACCESSION . VERSION . KEYWORDS MSP10-bio SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7938) AUTHORS Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, Rayner JC TITLE A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals New Vaccine Candidates and Protein-Protein Interactions. JOURNAL PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 10.1371/journal.pntd.0004264. eCollection 2015 Dec. PUBMED 26701602 REFERENCE 2 (bases 1 to 7938) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7938 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 154..533 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 534..737 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 688..708 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 734..758 /label=LNCX /note="Human CMV promoter, forward primer" primer_bind 1239..1258 /label=pMT2-F /note="Synthetic intron, forward primer" regulatory 1281..1290 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 3318..3373 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(3372..3391) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(5609..5627) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5695..5799 /gene="bla" /label=AmpR promoter CDS 5800..6660 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6018..6037) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6831..7419 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7320..7339 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7573..7590 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7707..7728 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7743..7773 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7781..7797 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7786..7808 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7805..7821 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7805..7821 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat 61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt 121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat 181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc 421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac 481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg 721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg 781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt 841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc 901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg 961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg 1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg 1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat 1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc 1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt 1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc 1321 tgctctgcat gtctggtgcg gccgcagctg tgcatgtgtc tgccaacgaa ctgaacggcg 1381 ccgatggcgg cgacgtgccc aaccagaagg acatcaccaa ggactacagc atcttcgagc 1441 ggatcaccag cgagggccag tctgccagcg gcaaggacga tagccctatc agccagagca 1501 acacccccca gggcgaagag gcctccgatg gcaagcaggg caataccccc ctggacgaga 1561 agaactccgc caaggacgtg gaagcccact tcatccggga aggccacgac aaagtgaccc 1621 acagcgacgt gggcaccgag gaaggcaaag ccacaggcca cgtgcagaag aacgccaacc 1681 tgcggagcac cagctacttc agtacgcagg gcgccgtgtc ccaggcctac aacttcgtgc 1741 aggaaaacca cccccagctg gacaacaacg gcgccaacgt ggaacaggtg gccagaggcc 1801 aggatgatgt gggcagcacc gagaacggcg agggcaattc tggcggcgag ggaaatcctc 1861 tgggcagcga taagcctggc ggcaagcctg aggacgccag caaggatacc cccggcaaca 1921 accccgagga aagccccaac cggaaccagg aaaagggcga gaaagagaag aaagagcgcg 1981 gcgacaccga caaccccaac agaggcaaga ccagcgagaa ggccgatcag cagggcggca 2041 accaccctaa tggcctgagc cccgatgaga agaacaaccc taagacccac aacaaccacg 2101 ccgagagcat caccaacccc ggggatgtgg gagccctgga cggcgaagaa aatggcgagg 2161 gcgacgacca gaccggcatc agccctacag aggaacaccc tacaggcgac gcccctccta 2221 gcgaccatgc cgagaagatc aagaacaccc tgctgaaaga gggcatcgac ctgaaagaaa 2281 ccaccagcat gatcgacaac gccgtgtaca acatggaaca gttcatcctg aaaacaaagt 2341 tctacaccac cgccatccgg aatttcgtgc acttcaaagt gaatcacatc tgcgagtaca 2401 gcaagtgcgg cgccaatgcc cggtgctaca tcgtggaaaa ggacaaagag gaatgccgct 2461 gccgggccaa ctacatgccc gacgatagcg tggactactt caagtgcatc cccatggtgg 2521 aaaaagactg cagcaaagag aacggcaact gcgacgtgaa cgccgagtgc agcatcgaca 2581 agaacaagga tatcaagtgc cagtgcaagt tcaactatat cggcgacggc atcttctgcg 2641 tgatgggctc tggcgcgccg tcgacctcca tcacggccta taagagtgag ggggagtcag 2701 cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag ttgagatgga 2761 aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag aaccaaaagg 2821 tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg ctcccactca 2881 cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg accctgactc 2941 tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg actcagcccg 3001 acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg agactgatct 3061 tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt caagtgcagg 3121 cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc aagatggact 3181 ccaagatcca ggttttatcc aaagggttga attccggatc actgcatcat attctggatg 3241 cacagaaaat ggtgtggaat catcgttaag tctagacacg tgattaatta aggatccccc 3301 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt 3361 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg 3421 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc 3481 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc 3541 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac 3601 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc 3661 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg 3721 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac 3781 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca 3841 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat 3901 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc 3961 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata 4021 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta 4081 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc 4141 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt 4201 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa 4261 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac 4321 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat 4381 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat 4441 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc 4501 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga 4561 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac 4621 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat 4681 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat 4741 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc 4801 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat 4861 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag 4921 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc 4981 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg 5041 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg 5101 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg 5161 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg 5221 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg 5281 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta 5341 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta 5401 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta 5461 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta 5521 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa 5581 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat 5641 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 5701 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 5761 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 5821 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 5881 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 5941 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 6001 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag 6061 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 6121 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 6181 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 6241 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 6301 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg 6361 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 6421 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 6481 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 6541 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 6601 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 6661 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 6721 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 6781 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 6841 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 6901 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 6961 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 7021 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 7081 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 7141 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 7201 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 7261 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 7321 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 7381 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 7441 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 7501 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 7561 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 7621 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 7681 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 7741 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 7801 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc 7861 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc 7921 agaactggta ggtatgga //