LOCUS       Exported                9132 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses full-length P. vivax P92 ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    P92-bio
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    4512..4567
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4566..4585)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6803..6821)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6889..6993
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6994..7854
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7212..7231)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8025..8613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8514..8533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8767..8784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8901..8922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8937..8967
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8975..8991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8980..9002
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8999..9015
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8999..9015
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcagatg tgtctctggc cggcgagttt cagagcaagc
     1381 tggcctatga cggcggccag atcgtgtaca gcaagaagcc cttcaagggc agcatctacg
     1441 ccttcagccc catcaagggc aagtgcgcca tcagcgtgaa ggccaccacc gacggcgtgg
     1501 aatgggagag caagagcaac atcgaggtgg aagatggcgt gggcggagcc accatcttca
     1561 gcctgttcac caccgatgag atcaccgcca tctttcggtg cagcaacgtg tactatatcg
     1621 ccaagatggg cggcgagaac ttctggaaga acatcaaaca agtgaccttc agcgacttcc
     1681 ccgtggacgc catccctgcc gtgtactctg gcagctacct gctgatgaac agcgagtacg
     1741 agaagttcat cctcgtgtgc agcagagagg accccaacgg cgccgagaga agctccgatg
     1801 gcgaagtgaa ggccgtgcct ttcagcgaac tgcagctgct gggccggtgc aagttcagct
     1861 tcgacgaggg caacacctgg aaagagggcg ccacccggat gtacagcgac gagctgagca
     1921 acgacgacgg cggcgaggat agcacagagc tgagcggcga caatacctgg gagagcgtga
     1981 aagaagtgaa tatcgccgcc gaagaggacg tgaagctgga agatcaagtg aaagagaagt
     2041 cccagcaggg cgctccccct agccatgcca cacagctgag cctgacacag atgggaggaa
     2101 agctggccat caaggccgtg aacgtgcgga atggcggcag cagcagcaag accgtgatcc
     2161 tcgtgtgtaa cagcctgtac aagaacgacc tgtactgcga caagatgaag ctgcagatca
     2221 acgacaagta catcctgcac acctacgaag tcgtgaacgg ctaccacatg gccgtgctga
     2281 gagagaagaa cggcgcccac ctggtgccca gcttcgtgta caatctggcc gagacattcg
     2341 agcctatcct gcactacaag tccgagaaga accgcgaggg caagagcgac gtgggcgagg
     2401 gaaaccctct ggacaaggcc gcctacgagt tcgtgtctgc caagggcgag gtggtgtacc
     2461 tgttctgcca caccgtgggc ggccacaaca aagtgatcaa gatcaacgcc cccgagcgga
     2521 agcctggctg cgaagtgctg cctgatggcg aggccaacaa gggctacagc cacaccttca
     2581 gcatccagaa catgcagaac gagcggatct gcaaggtgcc caccagcgag ctgggaacaa
     2641 cccaggacgg cttcttcaag gtgttctacg tgaccctgcc caagggcgtg tccctgaacg
     2701 gcgactgctt cacctttagc ttcagcaaag agctgaagtc cgagtaccac accgccgtcg
     2761 tgcggagagc caatggggga tctgctcctc acgaaggcga ggcccagttc tacttccccc
     2821 tgtactactc caagttcctg tacaactaca agaaaaccta ctgcaccctg tccaacggcc
     2881 tgaagatcgc cgtggaattc gactacatcc ggaacatcct ggacctgaac tacgccgacg
     2941 agaacgacgt gatcaaaatc ggctccaacg acgccgtggt gcacgccaag agaagatacg
     3001 tgcaggaaaa gggcggcgac gtgttcccca gaggcaccta cctgaactcc tacctgccct
     3061 tcgagaaaga atacgtgatc agcaacttcg tgcctgaggg cgtgaaccag gccagcagca
     3121 ttgtgctgcc acatggcgcc gtgcggaagc tgcattttgt gcagggcgga gaggtgctgc
     3181 cctacgacgg aattgacctg ctggccatta gccccaatta caagaagctg accaaatacg
     3241 agatgaaccg gaagtccatc gatgtgatcg tgtccaactt caagagcgag gaaaagatca
     3301 tcggcctcgt gtgccccgtg tccagccagg atgagggcct gacctgcttc gacaaagtgt
     3361 acctgcagaa gggcggcaga ctgcacaaca tcggctttgt gttcggcagc aacgacatct
     3421 tcgtgatccc ccagcggcgg ctgctgaggc ctggcggaaa agaagccatg gaaagcctgc
     3481 tgtacctgaa caagcggaac gtggacaagc tgaagaaaga gaaacagatc atccacttca
     3541 gctgcgagtg caagggcggg gagggaacag aacccgtgca cgtgaactac tacgtgtccg
     3601 cccactacga ctacgagttt gtgaaggccc aagtgggcaa gccaggcggc agcgcctttg
     3661 tgaatggaca cctggtggaa cagcggggcg aggccggaag agaaggcaca cctggcaaga
     3721 cagccgacag cgccgatctg accatctccg acgaggatct gaatctggaa gagggcctgc
     3781 ccatgtttgg ccagaacgtg ctgctgacca tgctgggcgg aggcagctct aagggacatc
     3841 ctcatggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     3901 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     3961 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     4021 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     4081 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     4141 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     4201 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     4261 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     4321 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     4381 tccaggtttt atccaaaggg ttgaattccg gatcactgca tcatattctg gatgcacaga
     4441 aaatggtgtg gaatcatcgt taagtctaga cacgtgatta attaaggatc ccccgacctc
     4501 gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     4561 tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc ctcggagatc
     4621 tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     4681 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     4741 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     4801 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     4861 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     4921 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     4981 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     5041 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     5101 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     5161 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     5221 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     5281 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     5341 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     5401 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     5461 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     5521 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     5581 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     5641 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     5701 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     5761 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     5821 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     5881 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     5941 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     6001 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     6061 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     6121 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     6181 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     6241 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     6301 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     6361 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     6421 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     6481 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     6541 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     6601 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     6661 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     6721 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga
     6781 gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6841 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6901 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6961 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7021 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7081 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7141 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7201 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     7261 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7321 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7381 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7441 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7501 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     7561 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7621 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7681 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7741 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7801 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7861 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7921 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7981 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8041 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8101 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8161 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8221 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8281 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8341 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8401 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8461 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8521 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8581 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8641 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8701 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8761 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8821 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8881 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8941 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     9001 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca attctcatgt
     9061 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
     9121 ggtaggtatg ga
//
