LOCUS       Exported                7455 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses full-length P. vivax RON12 ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    RON12-bio
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    2835..2890
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2889..2908)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5126..5144)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5212..5316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5317..6177
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5535..5554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6348..6936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6837..6856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7090..7107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7224..7245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7260..7290
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7298..7314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7303..7325
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7322..7338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7322..7338
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccctga gaaagtctaa agtggacggc ggagccatcg
     1381 gcatccaggg caaagaaaag aaaggccccg tggaaaaggc cagcggcgag gaagtgaaga
     1441 agaacggcgc cgagacaaac caggccagcg tgaaagtgtg cagcggcagc gattctaagg
     1501 gcggagccgg cgaaggctgt cctgtggaag cctctgctac atctggcgcc gagcagaccg
     1561 gcgagaagca ggatgtgggc aaggtgcaga acgtgggaaa agtgcagaat gtggaagtgc
     1621 agaacgtgga agtgcagaat gacgccagca acaagaagtc catcctggac agcgtcgtga
     1681 tccccgagga cagcttcgag agcgccaaga gcctgtgcga gttcgtcgtg attaagagcg
     1741 agcagttcaa ggccttctgc aagcagagca acctgttcaa agagatccag ctgctgaagc
     1801 agaaggaccc caacctgtac gcccagatcg ccggggagag ctacgagaat gaggccacca
     1861 gcagctttca ggtgctgaag gacgagggcg accggaagat cgtgatggaa gatgaccggc
     1921 acaaccccaa gatgtctata ttcttcctgt ttcaaaagat gtgcgtgggc ggcatccccc
     1981 tggcctgcac caatatcctg aaggtggaca acgtgttcga cgtgaaccag gctggcggac
     2041 agctgggcgg aggatctgcc ggaaaagccg aggccgaaga tgtggaaggc gagatcacag
     2101 gcgccgctga ggaacaggct agccagggcg cttctgagag cgtggccgat ctgacagagg
     2161 aaggatctgg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     2221 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     2281 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     2341 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     2401 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     2461 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     2521 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     2581 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     2641 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     2701 agatccaggt tttatccaaa gggttgaatt ccggatcact gcatcatatt ctggatgcac
     2761 agaaaatggt gtggaatcat cgttaagtct agacacgtga ttaattaagg atcccccgac
     2821 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     2881 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     2941 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     3001 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     3061 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     3121 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     3181 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     3241 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     3301 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     3361 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     3421 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     3481 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     3541 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     3601 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     3661 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     3721 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     3781 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     3841 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     3901 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     3961 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     4021 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     4081 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     4141 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     4201 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     4261 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     4321 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     4381 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     4441 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     4501 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     4561 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4621 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     4681 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     4741 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     4801 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     4861 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     4921 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     4981 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     5041 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     5101 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5161 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5221 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5281 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5341 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5401 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5461 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5521 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5581 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5641 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5701 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5761 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5821 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     5881 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5941 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6001 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6061 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6121 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6181 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6241 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6301 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6361 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6421 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6481 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6541 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6601 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6661 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6721 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6781 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6841 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6901 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6961 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7201 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7261 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7321 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     7381 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     7441 actggtaggt atgga
//
