LOCUS       Exported                7623 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses full-length P. vivax PVX_001015 ectodomain in mammalian 
            cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence .
ACCESSION   .
VERSION     .
KEYWORDS    PVX_001015-bio
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    3003..3058
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3057..3076)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5294..5312)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5380..5484
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5485..6345
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5703..5722)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6516..7104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7005..7024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7258..7275
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7392..7413
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7428..7458
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7466..7482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7471..7493
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7490..7506
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7490..7506
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcacaga acgagaacga agtgaccctg tgcctgagcg
     1381 agttcaacca ggacaaagaa tgcaacgtgg ccgccgagtt cggcaaagaa atgaaggtgt
     1441 actgccccat cgacagcgag cagaacaaca acggcggcgt cgtgcggatg aacggccggg
     1501 aagtgaacaa cgacaacgac tgcttcagca agatgaagtc caacgatgcc gccaaccgga
     1561 acgtggccaa gaaattcgcc gagtacgtgg aaaacctgat cctgcacacc aacgcccggg
     1621 acaacaccca ctacttctac gtgccccaca ccgtgacagc cggcgtgctg ctgagctgca
     1681 actgcatcga ccggcccaga cagaaggcct acaagctgaa catccagatc gccaagaatg
     1741 agaacaagaa cgtgaagggc tgcaacttct actacgccga cgagaacggg gagcagaacg
     1801 agcacagcgt ggaacagaat gtgaatgtga agaaaaagaa agtgtgcaat gtggacgtgc
     1861 aggccaacga cgtggtgtcc ttccggtgcc gggtgcacaa ccggtacagc aacgtgctcg
     1921 tgaacccccc cctgtgcttc cacgaggtgt ccaacgagga caaccagaaa acccagctgt
     1981 ctggcgtgct gaacggcgtg aaagtgatcc cccggatcgc cacctacgtg ccagacccta
     2041 tgatgccccg gttcctgagc tacctggtgg ccccacccca gatcaacgag gccctgaaag
     2101 tgaagtgcag ctgctccatc accgacaacg tgctggacat cagctacatc ggcaccatca
     2161 gcctgaactt catcaagacc gacaagctgc accccgtgaa agaagaggac gacgccgaga
     2221 agaaaaacaa gtggaagaac agcatcaatg agggcgaggt gcaggacggc ggcaacagcg
     2281 ataatagcgc ccccagagaa ggcgaggccg atgtgcgctc tgcctctgtg ctgacaggcg
     2341 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     2401 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     2461 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     2521 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     2581 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     2641 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     2701 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     2761 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     2821 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     2881 tatccaaagg gttgaattcc ggatcactgc atcatattct ggatgcacag aaaatggtgt
     2941 ggaatcatcg ttaagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg
     3001 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     3061 cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag
     3121 aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg
     3181 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc
     3241 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct
     3301 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc
     3361 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
     3421 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
     3481 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
     3541 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta
     3601 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat
     3661 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga
     3721 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt
     3781 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg
     3841 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg
     3901 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg
     3961 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa
     4021 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt
     4081 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac
     4141 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga
     4201 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca
     4261 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa
     4321 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca
     4381 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact
     4441 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat
     4501 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct
     4561 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt
     4621 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata
     4681 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat
     4741 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4801 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4861 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     4921 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac
     4981 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta
     5041 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     5101 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     5161 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     5221 atctgtatcc gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct
     5281 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5341 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5401 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5461 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5521 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5581 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5641 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5701 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     5761 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5821 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5881 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5941 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6001 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     6061 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6121 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6181 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6241 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6301 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6361 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6421 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6481 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6541 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6601 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6661 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6721 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6781 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6841 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6901 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6961 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7021 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7081 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7141 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7201 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7261 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7321 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7381 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7441 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7501 tatgaccatg attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct
     7561 tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat
     7621 gga
//
