LOCUS Exported 841 bp ds-DNA linear SYN 02-JUN-2021 DEFINITION mammalian expression of A2A receptor with N terminal TAP tag and C terminal YFP fusion. ACCESSION . VERSION . KEYWORDS N-TAP A2A-YFP pcDNA3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 841) AUTHORS Bergmayr C, Thurner P, Keuerleber S, Kudlacek O, Nanoff C, Freissmuth M, Gruber CW TITLE Recruitment of a cytoplasmic chaperone relay by the A2A adenosine receptor. JOURNAL J Biol Chem. 2013 Oct 4;288(40):28831-44. doi: 10.1074/jbc.M113.464776. Epub 2013 Aug 21. PUBMED 23965991 REFERENCE 2 (bases 1 to 841) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 2, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..841 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(14..733) /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(59..80) /label=EGFP-C /note="EGFP, forward primer" primer_bind 408..427 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 667..688 /label=EGFP-N /note="EGFP, reverse primer" regulatory 730..739 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS complement(785..802) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" ORIGIN 1 tcgagcggcc gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca 61 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact 121 ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct 181 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt 241 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt 301 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct 361 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc 421 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga 481 agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa gcactgcagg ccgtagccga 541 aggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca 601 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc 661 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc 721 ccttgctcac catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagaa 781 ttctatggtg atggtgatga tgaccggtat gcatattcag atcctcttct gagatgagtt 841 t //