LOCUS Exported 1063 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS GFP-rab11 WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1063) AUTHORS Choudhury A, Dominguez M, Puri V, Sharma DK, Narita K, Wheatley CL, Marks DL, Pagano RE TITLE Rab proteins mediate Golgi transport of caveola-internalized glycosphingolipids and correct lipid trafficking in Niemann-Pick C cells. JOURNAL J Clin Invest. 2002 Jun . 109(12):1541-50. PUBMED 12070301 REFERENCE 2 (bases 1 to 1063) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1063 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 48..57 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 54..770 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 54..770 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(99..120) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(360..379) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 707..728 /label=EGFP-C /note="EGFP, forward primer" misc_feature 771..836 /label=MCS /note="multiple cloning site" CDS 840..866 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 867..893 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" ORIGIN 1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga 61 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 121 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc 181 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 241 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 301 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 361 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 421 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 481 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca 541 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 601 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 661 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 721 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca 781 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccatgt 841 acccatacga tgttccggat tacgcttacc catacgatgt tccggattac gctggtaccc 901 gcgacgacga gtacgactac ctctttaaag ttgtccttat tggagattct ggtgttggaa 961 agagtaatct cctgtctcga tttactcgaa atgagtttaa tctggaaagc aagagcacca 1021 ttggagtaga gtttgcaaca agaagcatcc aggttgatgg aaa //