LOCUS Exported 712 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION 3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation. ACCESSION . VERSION . KEYWORDS pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 712) AUTHORS Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, Bamberg E, Nagel G, Gottschalk A, Deisseroth K TITLE Multimodal fast optical interrogation of neural circuitry. JOURNAL Nature. 2007 Apr 5. 446(7136):633-9. PUBMED 17410168 REFERENCE 2 (bases 1 to 712) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..712 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 411..420 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 agggttgctc aggcggataa ggatgacagg acaagtgagc agccactctg catagcgcag 61 ccactgcacc cggtgtcctg tggcaaggta gagcatagag ggattcttaa actcaaaaaa 121 gaactcgaga atcaccttaa ccatttcaat ggcgcacaca tagatctcct cccagccgca 181 tgtagatttc caggtttggt aggcatagaa catcagcagc aaaatgctga atcctgctgc 241 aagccactgc aggacatttg acgcggtctg agcgccgttc gtgccgcgag attcaatcca 301 tccggcacag taacattgat cctcagggac caggacggac ccgttcacca ccacaggatt 361 agtaacgaac aaaagttcgc gtccgacggc agacaaagcg ccgccatagt ccatggtggc 421 ctaccggtac ccggggatcc cccgctgccc ccagaactag gggccactcg cctgcccgtg 481 ctcctgagtg caaacggaga accggcttga ctgacgagct tggggcttct gagcagggca 541 ctgtggctgc tcacagcctc gcgagccttc gtcagcatcc aggtccccat ggcaaccacc 601 tccgaaacgc cctgttcccg ttgccccggt aactgcctcc ccaacacctg cctgcctttc 661 actttaaaac ctgctcctct ttgccctggc cttatataca caatactgca gg //