LOCUS       Exported                 733 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  3rd gen lentiviral expression of humanized ChR2 with H134R mutation 
            fused to mCherry driven by CaMKIIa promoter for optogenetic 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, 
            Bamberg E, Nagel G, Gottschalk A, Deisseroth K
  TITLE     Multimodal fast optical interrogation of neural circuitry.
  JOURNAL   Nature. 2007 Apr 5. 446(7136):633-9.
  PUBMED    17410168
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(325..344)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     594..612
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcttac ttgtacagct
       61 cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt
      121 agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct
      181 gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct
      241 tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct
      301 cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt
      361 tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg
      421 tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
      481 ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc
      541 cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca
      601 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
      661 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga
      721 tgatggccat gtt
//
