LOCUS Exported 990 bp ds-DNA linear SYN 11-JUN-2021 DEFINITION Standard for cell-free expression benchmarking.. ACCESSION . VERSION . KEYWORDS pCellFree_G03 CYP3A4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 990) AUTHORS Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K TITLE Performance benchmarking of four cell-free protein expression systems. JOURNAL Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. PUBMED 26301602 REFERENCE 2 (bases 1 to 990) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 11, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..990 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(20..43) /codon_start=1 /product="8xHis affinity tag" /label=8xHis /translation="HHHHHHHH" primer_bind complement(186..205) /label=T7 /note="T7 promoter, forward primer" promoter complement(187..205) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 237..341 /gene="bla" /label=AmpR promoter CDS 526..904 /label=ISS /note="immunostimulatory sequence from the AmpR gene; contains unmethylated CpG dinucleotides in the context of 5'-AACGTT-3' (Sato et al., 1996)" primer_bind complement(560..579) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 tcccgccctt gcttgtgccg tggtgatggt ggtgatggtg gtgaccagag gtgcccatgg 61 tttcacttac gtgtttaatg tctttacaga ctttatacat tactgtcatg gttaaataaa 121 ataaaataaa ataaaataaa ataaaataaa ataaaataaa ataaaataaa ataaacttaa 181 gatgtcccta tagtgagtcg tattagacgt caggtggcac ttttcgggga aatgtgcgcg 241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 961 actggatgga ggcgggataa agttgcagga //