LOCUS       Exported                 941 bp ds-DNA     linear   SYN 25-JUN-2021
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    pCellFree_G03 FYN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg tacagatttt caccaggttg gtactggggc tctgtcgcgg taaagtagtc
      241 ttccaggaag ctctgcaagt actcaaaagt ggggcgttct tcagggtcct ttttccagca
      301 gtggatcatg agctcatgca gagagatggg gcagtcctgc gggcagggca tcctgtagcc
      361 tcgctccacc tgctccagca cctcccggtt gttcatgcct gggtatggca ctcttccttt
      421 ggtgaccagc tctgtgagta agattccaaa agaccacacg tcagacttga ttgtgaacct
      481 cccgtacagg gctgcctcgg gggccgtcca cttgatgggg aactttgcac cttgtcttgc
      541 tgtgtactca ttgtcttcta tcaatcgggc caatccgaag tcagcaatct tgcatatgag
      601 tccattcccc actagaatgt ttgctgatcg cagatctcta tggatataat tcatgcgctc
      661 gatgtaagcc attcctgcag ccacctgtgc tgccatgtcc acaagatttg gtaatttcag
      721 agctcttcct tctccatctt ttaagaaatc cagtaaactt cctttgttca tatactcggt
      781 gacgatgtag atgggctcct cagacaccac tgcatagagc tggaccagct tgtcgtgctt
      841 cagcttcttc atgatctgcg cttcctcaag gaatgattcg ggggacattg tgcctggttt
      901 aagagtcttt atggctactt ttgtgtttcc attccaggta c
//
