LOCUS       Exported                 786 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  luciferase reporter for hA33 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    0.44 hA33 luciferase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Johnstone CN, White SJ, Tebbutt NC, Clay FJ, Ernst M, Biggs WH, 
            Viars CS, Czekay S, Arden KC, Heath JK
  TITLE     Analysis of the regulation of the A33 antigen gene reveals 
            intestine-specific mechanisms of gene expression.
  JOURNAL   J Biol Chem. 2002 Sep 13;277(37):34531-9. Epub 2002 Jul 11.
  PUBMED    12114523
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      479..488
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(551..569)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctatc gataggtacc agcaaatatg ggcaacaccc ttcttttact agcttggaac
       61 cctacctctg agtgcatttc cctttttatt atttatttcc tgtcagttat aagagaggcc
      121 tacccctttg tgagcagtct aggactttgt acacctgcta agtagggaga aggcagggga
      181 ggtggctggt ttaaggggaa cttgagggaa gtagggaaga ctcctctcgg gacctttgga
      241 gtaggtgaca catgagccca gccccagctc acctgccaat ccagctgagg agctcacctg
      301 ccaatccagc tgaggctggg cagaggtggg tgagaagagg gaaaattgca gggacctcca
      361 gttgggccag gccagaagct gctgtagctt taaccagaca gctcagacct gtatggaggc
      421 tgccagtgct cgagatctgc gatctaagta agcttggcat tccggtactg ttggtaaagc
      481 caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga
      541 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
      601 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat
      661 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
      721 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
      781 agttgc
//
