LOCUS Exported 729 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation. ACCESSION . VERSION . KEYWORDS pML107 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 729) AUTHORS Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, Wyrick JJ TITLE New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae. JOURNAL Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21. PUBMED 26305040 REFERENCE 2 (bases 1 to 729) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..729 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 105..114 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 cttttatagt tagtcttttt tttagtttta aaacaccaag aacttagttt cgaataaaca 61 cacataaaca aacaaaagat ctgatatgga tcgaattaga tctcgccacc atggacaaga 121 agtattctat cggactggac atcgggacta atagcgtcgg gtgggccgtc atcactgacg 181 agtacaaggt gccctctaag aagttcaagg tgctcgggaa caccgaccgg cattccatca 241 agaaaaatct gatcggagct ctcctctttg attcagggga gaccgctgaa gcaacccgcc 301 tcaagcggac tgctagacgg cggtacacca ggaggaagaa ccggatttgt taccttcaag 361 agatattctc caacgaaatg gcaaaggtcg acgacagctt cttccatagg ctggaagaat 421 cattcctcgt ggaagaggat aagaagcatg aacggcatcc catcttcggt aatatcgtcg 481 acgaggtggc ctatcacgag aaatacccaa ccatctacca tcttcgcaaa aagctggtgg 541 actcaaccga caaggcagac ctccggctta tctacctggc cctggcccac atgattaagt 601 tcagaggcca cttcctgatc gagggcgacc tcaatcctga caatagcgat gtggataaac 661 tgttcatcca gctggtgcag acttacaacc agctctttga agagaacccc atcaatgcaa 721 gcggagtcg //