LOCUS Exported 877 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven. ACCESSION . VERSION . KEYWORDS pL-CRISPR.EFS.tRFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 877) AUTHORS Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL TITLE Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. JOURNAL Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. PUBMED 24952903 REFERENCE 2 (bases 1 to 877) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..877 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(37..93) /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS complement(109..156) /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" ORIGIN 1 gttctcctta atcagctctt cgcccttaga caccatgggg ccggggttct cctccacgtc 61 gccggcctgc ttcagcaggc tgaagttggt ggcgccgctg ccgctagctt tctttttctt 121 agcttgacca gctttcttag tagcagcagg acgcttgtcg cctcccagct gagacaggtc 181 gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt ccagcacctc 241 tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga aggcggcagg 301 ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct ctctgatggg 361 cttatcccgg tgcttgttgt aggcggacag cactttgtcc agattagcgt cggccaggat 421 cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt gcttgtgctg 481 ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct tctcatagtg 541 gctggccagg tacaggaagt tcacatattt ggagggcagg gccagttcgt ttcccttctg 601 cagttcgccg gcagaggcca gcattctctt ccggccgttt tccagctcga acagggagta 661 cttaggcagc ttgatgatca ggtccttttt cacttctttg tagcccttgg cttccagaaa 721 gtcgatggga ttcttctcga agctgcttct ttccatgatg gtgatcccca gcagctcttt 781 cacactcttc agtttcttgg acttgccctt ttccactttg gccaccacca gcacagaata 841 ggccacggtg gggctgtcga agccgccgta cttctta //