LOCUS Exported 899 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Standard for cell-free expression benchmarking.. ACCESSION . VERSION . KEYWORDS pCellFree_G03 PC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 899) AUTHORS Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K TITLE Performance benchmarking of four cell-free protein expression systems. JOURNAL Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. PUBMED 26301602 REFERENCE 2 (bases 1 to 899) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..899 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 16..39 /codon_start=1 /product="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" /label=HRV 3C site /translation="LEVLFQGP" protein_bind 40..64 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 gacgagctat acaagctgga ggtgctcttc cagggtccga caagtttgta caaaaaagtt 61 ggcatgctga agttccgaac agtccatggg ggcctgaggc tcctgggaat ccgccgaacc 121 tccaccgccc ccgctgcctc cccaaatgtc cggcgcctgg agtataagcc catcaagaaa 181 gtcatggtgg ccaacagagg tgagattgcc atccgtgtgt tccgggcctg cacggagctg 241 ggcatccgca ccgtagccat ctactctgag caggacacgg gccagatgca ccggcagaaa 301 gcagatgaag cctatctcat cggccgcggc ctggcccccg tgcaggccta cctgcacatc 361 ccagacatca tcaaggtggc caaggagaac aacgtagatg cagtgcaccc tggctacggg 421 ttcctctctg agcgagcgga cttcgcccag gcctgccagg atgcaggggt ccggtttatt 481 gggccaagcc cagaagtggt ccgcaagatg ggagacaagg tggaggcccg ggccatcgcc 541 attgctgcgg gtgttcccgt tgtccctggc acagatgccc ccatcacgtc cctgcatgag 601 gcccacgagt tctccaacac ctacggcttc cccatcatct tcaaggcggc ctatgggggt 661 ggagggcgtg gcatgagggt ggtgcacagc tacgaggagc tggaggagaa ttacacccgg 721 gcctactcag aggctctggc cgcctttggg aatggggcgc tgtttgtgga gaagttcatc 781 gagaagccac ggcacatcga ggtgcagatc ttgggggacc agtatgggaa catcctgcac 841 ctgtacgagc gagactgctc catccagcgg cggcaccaga aggtggtcga gattgcccc //