LOCUS       Exported                 912 bp ds-DNA     linear   SYN 10-JUN-2021
DEFINITION  3' entry 2A-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    p3E-2a-tdTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(66..190)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             201..257
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(391..409)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     659..678
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     853..876
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gctcgggccc tgcagctcta gagctcgaat tctacaggtc actaatacca tctaagtagt
       61 tggttcatag tgactgcata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
      121 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca actttcttgt
      181 acaaagtgga cggatccgga gccacgaact tctctctgtt aaagcaagca ggagacgtgg
      241 aagaaaaccc cggtcctatg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct
      301 tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
      361 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
      421 tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga
      481 agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg
      541 agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc
      601 aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc
      661 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg
      721 acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc agccactacc
      781 tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact
      841 acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      901 acgagcgctc cg
//
