LOCUS       Exported                3799 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  3' entry clone with activated Dusp6 (S159/167A).
ACCESSION   .
VERSION     .
KEYWORDS    p3E-Dusp6DM_S159/197A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND
  TITLE     Vegfa signals through ERK to promote angiogenesis, but not artery 
            differentiation.
  JOURNAL   Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30.
  PUBMED    27578780
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..748)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1908..2003)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2040..2059)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2041..2059)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2064..2080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2064..2080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2182..2201)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2193..3002
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2260..2279)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3149..3737
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3638..3657
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtgggag gatcaggaat gctcgataag ttcaaacccg
      781 tgcagatcga ttcggtcatg gccataagca agacggtaga gtggctgaag gagcagctag
      841 aaacgcgcag agactgtttg ctcgttatgg actgccgagc gcaagagctg tacgagtcgt
      901 cgcacgtcga aacggccatt aacgtggcca tcccgagcct catgctccgg cgactcaaga
      961 aaggcaacct gcccatcaag tctctgcttt ctaacgggga agacagggaa aggttcgcgc
     1021 ggagatgcaa gacggacacg atcgtgttgt acgacgagta cagccgcgag tggaatgaaa
     1081 acatcgacgg cggctccgtg ttgggtttac tgctgaggag aatgaaggac gagggctaca
     1141 aggctttcta tctcgagggt ggcttcagca aatttcaaac tgattttccc gcaatgtgtg
     1201 agacgaacct cgacggttcg tcaagcagca gtgcaccaac ctcccaggtc ctgggtctcg
     1261 gagggctcag aatcagctct gactcctcgg acatcgagtc ggatatcgac cgagacccaa
     1321 gcagtgcaac tgactcagat ggcgcacccc tttccaaccc ccagccctca ttccccgtgg
     1381 agatccttcc acatctgtat ctgggctgtg ctaaggactc cacaaacctg gatatcctgg
     1441 aggagtttgg catcaagtac atcttgaacg tgacccctaa tctccccaac atgttcgaga
     1501 atgccgggga gtttaagtac aagcagattc ccatctctga tcactggagc cagaacctct
     1561 cacagttttt ccctgaagcc atcagcttta ttgatgaggc ccgtggactg aagtgtggcg
     1621 tgcttgttca ctgccttgca ggcatcagtc gttctgtcac tgtaacagtg gcgtacctca
     1681 tgcagaagct caacctgtcc atgaacgatg cttatgacat tgtcaaaatg aagaagtcga
     1741 acatctcgcc caactttaac ttcatgggtc aactcttgga ctttgagcgc acgttaggac
     1801 tcaagagtcc gtgtgataac cgtgtgccgg ccccaaccca gccactgtac ttcaccacac
     1861 caaccaatca caacgttttt cagttggacc ctctcgagtc cacgtaacaa ctttattata
     1921 caaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     1981 cagtcaaaat aaaatcatta tttggagctc catggtagcg ttaacgcggc cgcgatatcc
     2041 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     2101 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     2161 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     2221 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     2281 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     2341 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     2401 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     2461 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     2521 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     2581 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     2641 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     2701 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     2761 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2821 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2881 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2941 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     3001 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     3061 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3121 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3181 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3241 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3301 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3361 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3421 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3481 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3541 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3601 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3661 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3721 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3781 ggccttttgc tcacatgtt
//
