LOCUS       Exported               23969 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    BBa_J72114-BBa_J72104
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23969)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 23969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..23969
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(25..873)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(947..1783)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        1929..2047
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             2111..3553
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             3565..4488
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             4537..5619
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             5634..6617
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             6796..7908
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     RBS             7916..7924
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             9190..9198
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             12210..12218
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             13523..13531
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      14129..14200
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      14216..14243
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             14317..17391
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENCPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(14337..14354)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14337..14353)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14346..14368)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      17407..17501
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(17522..18181)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     18096..18115
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(18442..18459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18576..19121)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(18656..18675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    19202..19226
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      22361..22388
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      22404..22475
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(22727..23011)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(22785..22804)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             23038..23916
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 gaattcatga gatctcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct
       61 gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc
      121 acattcggtc gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg
      181 acacactctg acctgtcgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg
      241 ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg
      301 acttttcagg ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt
      361 tggcggcaac gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa
      421 cagcaactca gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg
      481 accgataacc tgccgcgcct gcgccatccc catgctacct aagcgccagt gtggttgccc
      541 tgcgctggcg ttaaatcccg gaatcgcccc ctgccagtca agattcagtt tcagacgctc
      601 cgggcaataa ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc
      661 gtcagcatga atgtaaaaga gatcgccacg ggtaatgcga taagggcgat cgttgagtac
      721 atgcaggcca ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcagc
      781 aaagacatct tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa
      841 atcatctttg agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag
      901 ttgattattc gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc
      961 catcccgtcc ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg
     1021 aaaagtggtt actgtcgctg aatccacagc gataggcgat gtcagtaacg ctggcctcgc
     1081 tgtggcgtag cagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg
     1141 tcagtcccgt ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat
     1201 ccgccacggc atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca
     1261 agttgagacg tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga
     1321 gcagtaattg cataaacaag atttcgcgac tggcggtcga gggtaaatca ttttcccctt
     1381 cctgctgttc catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc
     1441 gccagtgaga cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga
     1501 gaaactgaaa tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat
     1561 gttcatacag atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat
     1621 agggctgccc attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa
     1681 aatcatgatg atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg
     1741 cgttaccaga cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc
     1801 gtcaacacgg cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa
     1861 accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag
     1921 catcacatca ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg
     1981 ccaatggccc attttcctgt cagtaacgag aaggtcgcga atacaggcgc tttttagact
     2041 ggtcgtaatg aaattcagca ggatggatct aacttaagtt gaaattaccc ccattaaatg
     2101 gatggcaaat atgactaaaa aaatttcatt cattattaac ggccaggttg aaatctttcc
     2161 cgaaagtgat gatttagtgc aatccattaa ttttggtgat aatagtgttt acctgccaat
     2221 attgaatgac tctcatgtaa aaaacattat tgattgtaat ggaaataacg aattacggtt
     2281 gcataacatt gtcaattttc tctatacggt agggcaaaga tggaaaaatg aagaatactc
     2341 aagacgcagg acatacattc gtgacttaaa aaaatatatg ggatattcag aagaaatggc
     2401 taagctagag gccaattgga tatctatgat tttatgttct aaaggcggcc tttatgatgt
     2461 tgtagaaaat gaacttggtt ctcgccatat catggatgaa tggctacctc aggatgaaag
     2521 ttatgttcgg gcttttccga aaggtaaatc tgtacatctg ttggcaggta atgttccatt
     2581 atctgggatc atgtctatat tacgcgcaat tttaactaag aatcagtgta ttataaaaac
     2641 atcgtcaacc gatcctttta ccgctaatgc attagcgtta agttttattg atgtagaccc
     2701 taatcatccg ataacgcgct ctttatctgt tatatattgg ccccaccaag gtgatacatc
     2761 actcgcaaaa gaaattatgc gacatgcgga tgttattgtc gcttggggag ggccagatgc
     2821 gattaattgg gcggtagagc atgcgccatc ttatgctgat gtgattaaat ttggttctaa
     2881 aaagagtctt tgcattatcg ataatcctgt tgatttgacg tccgcagcga caggtgcggc
     2941 tcatgatgtt tgtttttacg atcagcgagc ttgtttttct gcccaaaaca tatattacat
     3001 gggaaatcat tatgaggaat ttaagttagc gttgatagaa aaacttaatc tatatgcgca
     3061 tatattaccg aatgccaaaa aagattttga tgaaaaggcg gcctattctt tagttcaaaa
     3121 agaaagcttg tttgctggat taaaagtaga ggtggatatt catcaacgtt ggatgattat
     3181 tgagtcaaat gcaggtgtgg aatttaatca accacttggc agatgtgtgt accttcatca
     3241 cgtcgataat attgagcaaa tattgcctta tgttcaaaaa aataagacgc aaaccatatc
     3301 tatttttcct tgggagtcat catttaaata tcgagatgcg ttagcattaa aaggtgcgga
     3361 aaggattgta gaagcaggaa tgaataacat atttcgagtt ggtggatctc atgacggaat
     3421 gagaccgttg caacgattag tgacatatat ttctcatgaa aggccatcta actatacggc
     3481 taaggatgtt gcggttgaaa tagaacagac tcgattcctg gaagaagata agttccttgt
     3541 atttgtccca taataggtaa aagtatggaa aatgaatcaa aatataaaac catcgaccac
     3601 gttatttgtg ttgaaggaaa taaaaaaatt catgtttggg aaacgctgcc agaagaaaac
     3661 agcccaaaga gaaagaatgc cattattatt gcgtctggtt ttgcccgcag gatggatcat
     3721 tttgctggtc tggcggaata tttatcgcgg aatggatttc atgtgatccg ctatgattcg
     3781 cttcaccacg ttggattgag ttcagggaca attgatgaat ttacaatgtc tataggaaag
     3841 cagagcttgt tagcagtggt tgattggtta actacacgaa aaataaataa cttcggtatg
     3901 ttggcttcaa gcttatctgc gcggatagct tatgcaagcc tatctgaaat caatgcttcg
     3961 tttttaatca ccgcagtcgg tgttgttaac ttaagatatt ctcttgaaag agctttaggg
     4021 tttgattatc tcagtctacc cattaatgaa ttgccggata atctagattt tgaaggccat
     4081 aaattgggtg ctgaagtctt tgcgagagat tgtcttgatt ttggttggga agatttagct
     4141 tctacaatta ataacatgat gtatcttgat ataccgttta ttgcttttac tgcaaataac
     4201 gataattggg tcaagcaaga tgaagttatc acattgttat caaatattcg tagtaatcga
     4261 tgcaagatat attctttgtt aggaagttcg catgacttga gtgaaaattt agtggtcctg
     4321 cgcaattttt atcaatcggt tacgaaagcc gctatcgcga tggataatga tcatctggat
     4381 attgatgttg atattactga accgtcattt gaacatttaa ctattgcgac agtcaatgaa
     4441 cgccgaatga gaattgagat tgaaaatcaa gcaatttctc tgtcttaaaa tctattgaga
     4501 tattctatca ctcaaatagc aatataagga ctctctatga aatttggaaa ctttttgctt
     4561 acataccaac ctccccaatt ttctcaaaca gaggtaatga aacgtttggt taaattaggt
     4621 cgcatctctg aggagtgtgg ttttgatacc gtatggttac tggagcatca tttcacggag
     4681 tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt tacttggcgc gactaaaaaa
     4741 ttgaatgtag gaactgccgc tattgttctt cccacagccc atccagtacg ccaacttgaa
     4801 gatgtgaatt tattggatca aatgtcaaaa ggacgatttc ggtttggtat ttgccgaggg
     4861 ctttacaaca aggactttcg cgtattcggc acagatatga ataacagtcg cgccttagcg
     4921 gaatgctggt acgggctgat aaagaatggc atgacagagg gatatatgga agctgataat
     4981 gaacatatca agttccataa ggtaaaagta aaccccgcgg cgtatagcag aggtggcgca
     5041 ccggtttatg tggtggctga atcagcttcg acgactgagt gggctgctca atttggccta
     5101 ccgatgatat taagttggat tataaatact aacgaaaaga aagcacaact tgagctttat
     5161 aatgaagtgg ctcaagaata tgggcacgat attcataata tcgaccattg cttatcatat
     5221 ataacatctg tagatcatga ctcaattaaa gcgaaagaga tttgccggaa atttctgggg
     5281 cattggtatg attcttatgt gaatgctacg actatttttg atgattcaga ccaaacaaga
     5341 ggttatgatt tcaataaagg gcagtggcgt gactttgtat taaaaggaca taaagatact
     5401 aatcgccgta ttgattacag ttacgaaatc aatcccgtgg gaacgccgca ggaatgtatt
     5461 gacataattc aaaaagacat tgatgctaca ggaatatcaa atatttgttg tggatttgaa
     5521 gctaatggaa cagtagacga aattattgct tccatgaagc tcttccagtc tgatgtcatg
     5581 ccatttctta aagaaaaaca acgttcgcta ttatattagc taaggagaaa gaaatgaaat
     5641 ttggattgtt cttccttaac ttcatcaatt caacaactgt tcaagaacaa agtatagttc
     5701 gcatgcagga aataacggag tatgttgata agttgaattt tgaacagatt ttagtgtatg
     5761 aaaatcattt ttcagataat ggtgttgtcg gcgctcctct gactgtttct ggttttctgc
     5821 tcggtttaac agagaaaatt aaaattggtt cattaaatca catcattaca actcatcatc
     5881 ctgtcgccat agcggaggaa gcttgcttat tggatcagtt aagtgaaggg agatttattt
     5941 tagggtttag tgattgcgaa aaaaaagatg aaatgcattt ttttaatcgc ccggttgaat
     6001 atcaacagca actatttgaa gagtgttatg aaatcattaa cgatgcttta acaacaggct
     6061 attgtaatcc agataacgat ttttatagct tccctaaaat atctgtaaat ccccatgctt
     6121 atacgccagg cggacctcgg aaatatgtaa cagcaaccag tcatcatatt gttgagtggg
     6181 cggccaaaaa aggtattcct ctcatcttta agtgggatga ttctaatgat gttagatatg
     6241 aatatgctga aagatataaa gccgttgcgg ataaatatga cgttgaccta tcagagatag
     6301 accatcagtt aatgatatta gttaactata acgaagatag taataaagct aaacaagaga
     6361 cgcgtgcatt tattagtgat tatgttcttg aaatgcaccc taatgaaaat ttcgaaaata
     6421 aacttgaaga aataattgca gaaaacgctg tcggaaatta tacggagtgt ataactgcgg
     6481 ctaagttggc aattgaaaag tgtggtgcga aaagtgtatt gctgtccttt gaaccaatga
     6541 atgatttgat gagccaaaaa aatgtaatca atattgttga tgataatatt aagaagtacc
     6601 acatggaata tacctaatag atttcgagtt gcagcgaggc ggcaagtgaa cgaatcccca
     6661 ggagcataga taactatgtg actggggtga gtgaaagcag ccaacaaagc agcagcttga
     6721 aagatgaagg gtataaaaga gtatgacagc agtgctgcca tactttctaa tattatcttg
     6781 aggagtaaaa caggtatgac ttcatatgtt gataaacaag aaattacagc aagctcagaa
     6841 attgatgatt tgattttttc gagcgatcca ttagtgtggt cttacgacga gcaggaaaaa
     6901 atcagaaaga aacttgtgct tgatgcattt cgtaatcatt ataaacattg tcgagaatat
     6961 cgtcactact gtcaggcaca caaagtagat gacaatatta cggaaattga tgacatacct
     7021 gtattcccaa catcggtttt taagtttact cgcttattaa cttctcagga aaacgagatt
     7081 gaaagttggt ttaccagtag cggcacgaat ggtttaaaaa gtcaggtggc gcgtgacaga
     7141 ttaagtattg agagactctt aggctctgtg agttatggca tgaaatatgt tggtagttgg
     7201 tttgatcatc aaatagaatt agtcaatttg ggaccagata gatttaatgc tcataatatt
     7261 tggtttaaat atgttatgag tttggtggaa ttgttatatc ctacgacatt taccgtaaca
     7321 gaagaacgaa tagattttgt taaaacattg aatagtcttg aacgaataaa aaatcaaggg
     7381 aaagaccttt gtcttattgg ttcgccatac tttatttatt tactctgcca ttatatgaaa
     7441 gataaaaaaa tctcattttc tggagataaa agcctttata tcataaccgg aggcggctgg
     7501 aaaagttacg aaaaagaatc tctgaaacgt gatgatttca atcatctttt atttgatact
     7561 ttcaatctca gtgatattag tcagatccga gatatattta atcaagttga actcaacact
     7621 tgtttctttg aggatgaaat gcagcgtaaa catgttccgc cgtgggtata tgcgcgagcg
     7681 cttgatcctg aaacgttgaa acctgtacct gatggaacgc cggggttgat gagttatatg
     7741 gatgcgtcag caaccagtta tccagcattt attgttaccg atgatgtcgg gataattagc
     7801 agagaatatg gtaagtatcc cggcgtgctc gttgaaattt tacgtcgcgt caatacgagg
     7861 acgcagaaag ggtgtgcttt aagcttaacc gaagcgtttg atagttgagg atctttaagg
     7921 aggtaaaaaa aatgaaacat tcttccgata tctgcattgt tggtgctggt atttctggtt
     7981 tgacgtgcgc aagccatctg ctggacagcc cggcatgccg tggtctgagc ctgcgtatct
     8041 ttgacatgca gcaagaagcc ggtggccgta tccgcagcaa aatgctggat ggtaaggcaa
     8101 gcattgaact gggcgcaggt cgctactccc ctcagttgca cccgcatttc caaagcgcaa
     8161 tgcagcacta tagccaaaag agcgaagtct atccgttcac ccagttgaag ttcaaatctc
     8221 acgtgcagca aaagctgaag cgcgccatga atgaactgtc cccgcgtctg aaagagcatg
     8281 gtaaagagag ctttttgcag tttgtcagcc gttatcaagg tcacgatagc gcggttggta
     8341 tgatccgctc tatgggttac gacgcactgt tcctgccgga tatcagcgca gaaatggcct
     8401 acgacattgt gggtaagcac ccggagatcc agagcgtgac ggacaacgac gcgaaccaat
     8461 ggtttgcagc ggaaacgggc tttgctggtc tgattcaggg catcaaggct aaggttaagg
     8521 cggcaggtgc gcgttttagc ctgggttatc gtctgctgag cgtccgtacc gacggtgacg
     8581 gctacctgct gcaactggca ggtgacgacg gctggaaact ggagcaccgt acccgccatc
     8641 tgattctggc gattccgccg agcgcgatgg cgggtttgaa tgttgatttt ccagaagcct
     8701 ggtccggtgc gcgctatggc agcctgccgc tgtttaaggg ctttctgacg tacggtgagc
     8761 cgtggtggtt ggactacaaa ctggacgatc aggtgctgat tgttgacaac ccgctgcgca
     8821 aaatctattt caaaggcgat aagtacctgt tcttctatac cgatagcgag atggcgaatt
     8881 actggcgcgg ttgtgtcgcg gagggcgagg acggttacct ggagcaaatt cgcacccatt
     8941 tggctagcgc actgggtatc gtccgtgaac gtatcccgca accgctggca cacgttcaca
     9001 agtattgggc gcacggcgtt gagttttgcc gtgattctga tattgaccac ccgagcgcac
     9061 tgtctcatcg cgacagcggt atcatcgcgt gctccgatgc gtacacggag cattgtggtt
     9121 ggatggaggg cggtctgctg agcgcccgtg aggcaagccg tctgctgttg cagcgtatcg
     9181 ccgcgtgatt aaggaggtaa aaaaaatgag cattctggat ttcccgcgta tccacttccg
     9241 tggctgggcc cgtgtcaatg cgccgaccgc gaaccgcgat ccgcacggcc acatcgatat
     9301 ggccagcaat accgtggcga tggcgggtga gccgttcgac ctggcacgcc atcctacgga
     9361 gttccaccgt cacctgcgct ccctgggtcc gcgcttcggc ttggatggtc gtgctgaccc
     9421 ggaaggcccg ttcagcctgg ccgagggcta caacgctgcc ggtaacaacc acttttcgtg
     9481 ggagagcgca accgttagcc acgtgcaatg ggatggcggt gaggcggatc gtggtgacgg
     9541 tctggtcggt gctcgtttgg cactgtgggg tcactacaat gattatctgc gtaccacctt
     9601 caatcgtgct cgttgggtcg acagcgaccc gacgcgccgt gacgctgcac aaatctatgc
     9661 gggccaattc accattagcc cggctggtgc cggtccgggt acgccgtggc tgtttacggc
     9721 agacattgat gatagccatg gtgcacgttg gacgcgtggc ggccacattg cagagcgtgg
     9781 cggccacttc ttggatgaag agtttggtct ggcacgcctg tttcagttct ctgtgccgaa
     9841 agatcaccca cattttctgt ttcacccggg tccgtttgat tccgaggcct ggcgtcgtct
     9901 gcaattggct ctggaggatg acgacgttct gggtctgacc gtgcaatatg cgttgttcaa
     9961 tatgagcacc ccgcctcagc cgaacagccc ggtttttcac gatatggtcg gtgttgtcgg
    10021 tctgtggcgt cgtggtgaac tggcgagcta cccggctggt cgtctgctgc gtccgcgtca
    10081 accgggtctg ggtgacctga ccctgcgcgt caacggtggt cgcgttgcgc tgaatttggc
    10141 gtgtgccatt ccgttcagca ctcgtgccgc gcagccaagc gcaccggacc gcctgacccc
    10201 ggacctgggt gccaaactgc cgctgggcga tctgctgctg cgtgatgagg acggcgcact
    10261 gttggcacgt gtgccgcagg ctctgtacca agactattgg acgaatcacg gtattgtgga
    10321 cctgccgctg ctgcgcgaac cgcgtggtag cttgaccctg agcagcgaac tggcggagtg
    10381 gcgtgagcaa gactgggtca cccaaagcga cgcgtctaac ctgtacctgg aggcaccgga
    10441 tcgccgtcac ggtcgctttt tccctgagag catcgcgctg cgcagctact ttcgcggtga
    10501 agcgcgtgcg cgtccggata tcccgcatcg tatcgagggc atgggcctgg tcggcgtcga
    10561 atctcgtcag gatggcgacg ctgcggaatg gcgtctgacg ggtctgcgtc cgggtccggc
    10621 acgcattgtt ctggacgatg gtgccgaggc gatccctctg cgtgttctgc ctgacgattg
    10681 ggcgctggat gacgcgaccg tcgaagaagt ggattacgcc tttttgtacc gccacgttat
    10741 ggcgtattac gagctggtgt atccattcat gagcgacaag gtgttttccc tggctgatcg
    10801 ttgcaaatgt gaaacgtacg cacgtctgat gtggcagatg tgtgatccgc agaaccgcaa
    10861 caagtcctat tacatgccga gcacccgcga actgtcggca ccgaaagctc gtttgttctt
    10921 gaagtatctg gcccacgtgg aaggccaggc acgcctgcaa gcacctccgc cagcgggtcc
    10981 ggcacgcatt gaatctaaag cccagttggc ggcagagctg cgtaaagccg tcgacctgga
    11041 gctgtctgtg atgctgcaat acctgtacgc ggcgtatagc attccgaact atgcacaggg
    11101 ccaacaacgt gttcgtgacg gtgcgtggac cgccgagcag ctgcaactgg cgtgcggtag
    11161 cggtgaccgt cgccgtgatg gcggtattcg tgcagcactg ctggaaattg ctcatgaaga
    11221 aatgattcat tacctggtcg ttaacaacct gctgatggcc ctgggcgagc cgttctacgc
    11281 gggtgtcccg ctgatgggcg aagcggcacg tcaggcgttt ggcctggaca ccgagttcgc
    11341 tctggaaccg tttagcgaaa gcacgctggc acgttttgtt cgtctggaat ggccgcactt
    11401 tatcccagca ccgggcaaat ccatcgcgga ctgctatgcc gccattcgtc aggcgttttt
    11461 ggatctgccg gacttgtttg gtggcgaggc aggtaagcgt ggcggtgaac accacctgtt
    11521 cctgaatgag ctgaccaacc gtgcgcatcc gggttatcaa ctggaagttt tcgatcgcga
    11581 ctcggcgctg tttggtattg catttgtgac cgatcagggc gaaggtggcg ctctggacag
    11641 cccgcactac gaacatagcc attttcaacg tctgcgtgaa atgagcgcgc gtatcatggc
    11701 tcaaagcgca ccgttcgaac cggcgctgcc ggcgttgcgt aatccggttc tggatgagag
    11761 cccgggttgc caacgtgtcg cagacggtcg tgcgcgtgcg ctgatggcat tgtaccaagg
    11821 cgtttatgag ctgatgtttg cgatgatggc gcagcacttc gccgtgaaac cgctgggtag
    11881 cttgcgtcgc agccgcctga tgaacgcagc aatcgatctg atgaccggtc tgttgcgtcc
    11941 gctgagctgc gcgctgatga acctgccaag cggcatcgcc ggtcgcacgg ccggtccgcc
    12001 gctgccgggt ccggttgaca cccgtagcta tgacgactac gcgctgggct gtcgcatgct
    12061 ggcacgccgt tgcgagcgtc tgctggagca ggcgagcatg ctggaaccgg gttggctgcc
    12121 ggatgcgcag atggagctgc tggatttcta tcgtcgccaa atgctggact tggcgtgcgg
    12181 caaactgagc cgcgaggcct aatgatcctt aaggaggtaa aaaaaatgaa acgtgcgatt
    12241 atcgttggtg gcggcctggc gggtggcctg accgcgatct acctggcgaa gcgtggctac
    12301 gaagtgcacg tcgtggagaa gcgtggtgat cctctgcgcg atctgagctc ttacgtggac
    12361 gttgttagca gccgtgcgat cggcgtgagc atgaccgttc gtggtatcaa gagcgttttg
    12421 gctgcgggca ttccgcgtgc agagctggat gcgtgtggcg aaccgatcgt ggcaatggct
    12481 ttctccgtgg gtggtcagta tcgcatgcgc gaactgaagc cgttggagga tttccgtccg
    12541 ctgagcttga accgtgcggc gtttcaaaag ctgctgaaca aatacgcgaa cctggcaggc
    12601 gttcgttact actttgagca taagtgcctg gatgttgacc tggatggtaa gagcgtgttg
    12661 attcagggca aagatggtca gccgcagcgt ctgcaaggtg acatgattat cggtgcggat
    12721 ggcgcccaca gcgccgtccg tcaggcgatg cagagcggcc tgcgtcgttt cgagttccag
    12781 caaacgttct tccgccatgg ctacaaaacc ctggttttgc cggacgcgca agcactgggt
    12841 taccgtaaag acacgctgta ctttttcggc atggattccg gtggcctgtt cgcgggtcgt
    12901 gcggctacga tcccagatgg tagcgtcagc atcgccgttt gcctgccgta ctcgggtagc
    12961 ccttccctga cgaccaccga cgaaccgacg atgcgtgcgt tcttcgatcg ttacttcggt
    13021 ggcctgccgc gtgacgcgcg tgacgaaatg ctgcgtcagt ttctggcgaa gccgagcaac
    13081 gacctgatta acgtgcgctc tagcaccttt cactataagg gtaatgtgct gttgctgggt
    13141 gatgctgcgc atgcgactgc gccgttcctg ggtcagggta tgaacatggc gctggaggac
    13201 gcccgcacgt ttgtcgagct gctggaccgc caccagggcg accaagacaa agcctttccg
    13261 gagttcacgg agctgcgcaa agtccaggca gacgcaatgc aagacatggc tcgcgccaac
    13321 tatgacgttt tgagctgctc gaacccgatc tttttcatgc gtgcgcgtta cacgcgttac
    13381 atgcattcca agtttccggg cctgtatccg ccggatatgg ccgagaaact gtactttacg
    13441 agcgagccgt acgatcgtct gcaacaaatc cagcgtaaac agaatgtttg gtacaagatt
    13501 ggtcgcgtga attgatgatc tttaaggagg taaaaaaaat ggagaaccgt gagccaccac
    13561 tgttgccagc ccgttggagc agcgcctatg tctcttattg gagcccgatg ctgccggatg
    13621 accagctgac cagcggctat tgctggttcg actatgaacg tgacatctgt cgtattgacg
    13681 gcctgttcaa tccgtggagc gagcgtgata ctggttatcg cctgtggatg tcggaggttg
    13741 gtaatgcggc cagcggccgt acctggaaac aaaaagtcgc ctatggtcgt gagcgtaccg
    13801 ccctgggtga acagctgtgt gagcgtccgc tggatgatga gactggccct tttgccgaat
    13861 tgttcctgcc acgcgatgtc ctgcgccgtc tgggtgcccg tcacattggc cgtcgcgtgg
    13921 ttctgggtcg cgaagcggac ggttggcgtt accagcgccc aggtaaaggt ccgagcaccc
    13981 tgtacctgga tgcggcgagc ggcactccac tgcgcatggt caccggcgat gaagcgtcgc
    14041 gtgcaagcct gcgtgatttt ccgaatgtga gcgaggcgga gatcccggac gcggttttcg
    14101 cggccaagcg ctaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
    14161 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
    14221 accttcgggt gggcctttct gcgtttatag gatctgttaa tgctagctca gtcctaggga
    14281 ctctgctagc ggatctattt cactagggaa gccactatga ccatgattac ggattcactg
    14341 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    14401 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    14461 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
    14521 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
    14581 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
    14641 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
    14701 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
    14761 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
    14821 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
    14881 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
    14941 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
    15001 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc
    15061 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc
    15121 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
    15181 cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat
    15241 ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc
    15301 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
    15361 ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
    15421 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
    15481 cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg
    15541 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
    15601 gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat
    15661 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat
    15721 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa
    15781 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat
    15841 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct
    15901 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc
    15961 ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc
    16021 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct
    16081 tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc
    16141 tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc
    16201 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
    16261 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa
    16321 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag
    16381 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca
    16441 tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt
    16501 gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt
    16561 tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag
    16621 atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca
    16681 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc
    16741 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca
    16801 gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa
    16861 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
    16921 gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag
    16981 ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactgtccc
    17041 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
    17101 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
    17161 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg
    17221 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
    17281 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattt
    17341 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata aggatccgag
    17401 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
    17461 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
    17521 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
    17581 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
    17641 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
    17701 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
    17761 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
    17821 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
    17881 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
    17941 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
    18001 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
    18061 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
    18121 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
    18181 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
    18241 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaaagat ccgcaccgcc
    18301 ggacatcagc gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
    18361 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
    18421 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
    18481 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
    18541 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
    18601 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
    18661 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
    18721 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
    18781 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
    18841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
    18901 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
    18961 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
    19021 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
    19081 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
    19141 agataaaata tttctagagg cctcccctga ttctgtggat aaccgggatc tgtaaggatc
    19201 aaccactttg tacaagaaag ctgggtcgaa ttgagatccg aacggtttat tacgtacatc
    19261 aggtaaaact gaccgataag ccgctttctt ttgggtatag tgtcgtggac agtcattcat
    19321 ctttctgccc ctccaaaagt aaaaacccgc cgaagcgggt ttttacgtaa aacaggtgaa
    19381 actgaccgat aagccgcttt cttttgggta tagtgtcgtg gacagtcatt catctttctg
    19441 cccctccaaa agcaaaaacc cgccgaagcg ggtttttacg taaaccaggt gaaactgacc
    19501 gataagccgc tttcttttgg gtatagcgtc gtggacagtc attcatcttt ctgcccctcc
    19561 aaaagcaaaa acccgccgaa gcgggttttt acgtaaatca ggtgaaactg accgataagc
    19621 cgggttctgt cgtggacagt cattcatcta ggccagcaat cgccagatcc ttaatcatca
    19681 tccccgtaca gtttgcgggt aacttcttcg tcttcctcct cgtcttcgtc cagatcctgt
    19741 tcaagccatg cttcgtttga tatgccttcc gcatcgacat caccataacc acctgtatcg
    19801 acgatatcgg caatttcttc tctacgatgc tcaatccaca atgcggcatc agcgcggcgg
    19861 ctggcggccc gttctcgcgt gattttatcc agatcctcaa gtgatggagc gccagatgct
    19921 gtttggtttt cctcatcatc ggtattggtc ttaggagcac gcagatcggc tttgatttgc
    19981 tccagcatca ggggcggcac ttttcctcca tgcgcctcga tgaactcagc cgcttccagc
    20041 actgaccagt tattttcacg ctttcgttcg tatgccagct taacaatgcc agcttgcccc
    20101 atagataaag cgtgcttttc cgcctcccgg ctttcttttc gatagttatt ccggatgctg
    20161 taaatggtgt tgatcaggct gcttatctgc gcggaacagc tgtttagcat gctcgcgata
    20221 cggtattcag gcggagtacc ttcatcatcg tctttttgct gatcgcgcat ttcctgcacc
    20281 aggcgaatac acgtatccct ggcgttctcc agcataagga gatgagaaag agacttttcc
    20341 agaagagtgg tttccagaac atcggccccg gaccgacgca acatagcgcg cgcggccttc
    20401 cgcgcttcaa cgttatctat caggtaatcg ccaacttcga actcaaagcg ttcaccatca
    20461 tcatccaggg tgtcgcgttc caggcgatca cgtaaggtcc ggtgggcgcg ggtgatcacg
    20521 tcatgatcat ctgaacgatc atttatgcgc ttattttggc gcttcgcatt ctcgactgcg
    20581 gcactgacaa cggcattaac tctttgtttt tccgctattt cagccgcaat gtgatcacct
    20641 gcatgttgat cattagagtg atcaatgatc atgcttttta gtggcttcct gactggctta
    20701 tttggcttgc ggctgtccgt agtcctggtg tcttctttga aggcacggag ataacgacgt
    20761 gcggtattag ggttaagatt aaactcggcg gcatactgtg cgatggtgta accaccatct
    20821 cgcgccaggc gagcaaaatt cttcttgtga tcgtcccagg tcacttatgc ttcctttcga
    20881 gatcccaaca atattcctac tcaatgaaca aatgactact cgtagaatcg gttaacacac
    20941 cagattcaga tccttaccct ctgaatcctg ccggtatacc ccattgttcg ttatctttat
    21001 ttttggctaa aaccgcatta agagcttcgt ttaccgtcat gcaatgcggt aggttatcga
    21061 agtttgatat cccgccaata tcaggcgaac gcttgttttt caggtaagca tatttccgcg
    21121 cagccgcctc tactttctgc ttgaactcat gtttttgagt gcgttttttg gataaccgca
    21181 gattgtcagc ctttgctttt gccttagcga tccatgaagt caattttttg aggctggttg
    21241 ttccggcacc gccggaaact gatctttttg tttttttaac ttgtgacttc ttattcttta
    21301 ttgccacgtc atcctgacag ggggaggggg tatcattttg acatgggggt gtggataaaa
    21361 aattaaataa agccaatgtc ttagcgagaa cagctttaac cttggttgcc gctgacgaaa
    21421 tctttaattt gctttctatc agcgcatttt tggcttgttg tgcgaaggcc aaaaaggatg
    21481 gtgtaaaccg gtacaggtta gcgcgacgtt cacggtgatc gccgataaca atctctacag
    21541 acagaatacc tttgtttaca gcttcacgga atgcacgaac gacggttgat tggctataac
    21601 cagtttctgc cgcgatcagg cggtgaggct tgtgaatgaa gtattcactg gttgttgccg
    21661 cgagatttgc acattgcgac aggatatgcc cggcgctacg ggatagaccg gagtgtgtta
    21721 caaagcaggc caattcatag ccagaaaaag taaaatcgct ttaagatccc cgagttctct
    21781 taaaccaagg tttaggattg aaatgatgac gccggaaact tcttataaag cgtggaaaca
    21841 gccacatcat agatgattgc aacctgctta cgggggatgc ccttctccag caatcgccgc
    21901 atttgctgcc atgtttcttc ttggtattta ggccgacgcc cacctatacg accttctgcg
    21961 cgagctgcat caagtccagc gcgtgtacgt tcaacgataa gctcacgttc catttctgcc
    22021 agcgccccca ttacgtgaaa gaaaaagcgc cccattggtg tactggtgtc gatggagtca
    22081 gtgagactcc ggaagttaat gcctctgtca cgcagctctt ccaccagcac aactaagtga
    22141 cgcatgctgc gcccaagacg gtctaacttc catacgacca gggtatcacc tctggaaagt
    22201 tacggagtac cttttttaac ccagggcgct cagccttttt gccgctcgcc ttgtcctcaa
    22261 aaattagctc acatcctgcg ctttcaagag cgtttcgttg taaagcagtg ttttgttcat
    22321 ttgttgatac gcgtacatag cctattagag atcctataaa cgcagaaagg cccacccgaa
    22381 ggtgagccag tgtgactcta gtagagagcg ttcaccgaca aacaacagat aaaacgaaag
    22441 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggagatcct tattgtgagt
    22501 ctggctggtt agtggccatg agatattcga ttgtgtcagt gagatcatcc aggtcgtctt
    22561 gggtgatgcg gtactcctga ttggatatct ttgagtagtg ttcagcaatg gcgcgggcag
    22621 cgtcggtttc ggcggggtct acagataaag cgttagagca atgtctaacg tcgtcgatgg
    22681 ttggtggaat gaaagccata attatgcctc actgtaagat ccgggtatgg agaaacagta
    22741 gagagttgcg ataaaaagcg tcaggtaaga tccgctaatc ttatggataa aaatgctatg
    22801 gcatagcaaa gtgtgacgcc gtgcaaataa tcaatgtgga cttttctgcc gtgattatag
    22861 acacttttgt tacgcgtttt tgtcatggct ttggtcccgc tttgttacag aatgctttta
    22921 ataagcgggg ttaccggttt ggttagcgag aagagccagt aaaagacgca gtgacggcaa
    22981 tgtctgatgc aatatggaca attggtttct tctctgaatg gcgggagtat gaaaagtatg
    23041 gctgaagcgc aaaatgatcc cctgctgccg ggatactcgt ttaatgccca tctggtggcg
    23101 ggtttaacgc cgattgaggc caacggttat ctcgattttt ttatcgaccg accgctggga
    23161 atgaaaggtt atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga
    23221 cgagaatttg tttgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac
    23281 tacggtcgtc atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc
    23341 gcctactggc atgaatggct taactggccg tcaatatttg ccaatacggg gttctttcgc
    23401 ccggatgaag cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg
    23461 caaggggaag ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg
    23521 cggcgcatgg aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag
    23581 gcttgtcagt acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca
    23641 cagcatgttt gcttgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt
    23701 agcgtcttaa gctggcgcga ggaccaacgt atcagccagg cgaagctgct tttgagcacc
    23761 acccggatgc ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc
    23821 tcgcgggtat ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa
    23881 gaaaaagtga atgatgtagc cgtcaagttg tcataattgg taacgaatca gacaattgac
    23941 ggctcgatgg agtagcatag agatctcat
//
