LOCUS Exported 5767 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR. ACCESSION . VERSION . KEYWORDS pMM553 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5767) AUTHORS Mimee M, Tucker AC, Voigt CA, Lu TK TITLE Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. JOURNAL Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001. PUBMED 26918244 REFERENCE 2 (bases 1 to 5767) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5767 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 3767..4627 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3985..4004) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" oriT 4758..4867 /note="incP origin of transfer" rep_origin 5038..5426 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" ORIGIN 1 atggttttta ctctggaaga ttttgttggc gattggcgtc agaccgcggg ttataatttg 61 gatcaagtcc tggaacaggg tggcgtaagc tctctgttcc agaacctggg tgtgagcgtg 121 acgccgattc agcgcatcgt tctgtccggc gagaacggtc tgaaaattga tattcatgtg 181 atcatcccgt acgaaggcct gagcggtgac caaatgggtc aaatcgagaa aatctttaaa 241 gtcgtctacc cagttgacga tcaccacttc aaggttatct tgcattacgg tacgctggtg 301 attgatggtg tgaccccgaa tatgattgac tatttcggcc gtccgtatga aggcattgcc 361 gtttttgacg gtaaaaagat caccgtcacc ggtaccctgt ggaatggcaa taagattatt 421 gacgagcgtc tgattaaccc ggacggcagc ctgctgttcc gcgtgaccat caacggtgtc 481 acgggttggc gtctgtgcga gcgcatcctg gcataatgaa ctgcacttgc tttgataatt 541 aatgataaac aatctaaaag cactctaatc gttatcggag tgcttttaga ttactaatca 601 aattgcttct actaattgcc tatcttccag tgatggaaca gcatttgtgc attggctgca 661 acaatcagcc ttacttgtgc ctgttctatt tccgaaccga ccgcttgtat gaatccatca 721 aaattcgttt tctctatgtt ggattccttg ttgctcatat tgtgatgata atttctacaa 781 atatagtcat tggtaactat ctatgaaact gtttgatact tttatagttg attaaacttg 841 ttcatggcat ttgccttaat atcatccgct atgtcaatgt agggtttcat agctttgtag 901 tcgctgtgtc ccgtccattt catgaccacc tgtgccggga ttccgagagc cagcgcattg 961 cagatgaatg tccttcttcc tgcatgggta ctgagcaaag cgtatttggg tgtgacttca 1021 tcaatacgtt catttccctt gtagtaggtt tcccgtacag gctcgttgat ttctgccagt 1081 tcgcccagct ctttcaggta atcgttcatc ttctggttgc tgatgacggg cagagccatg 1141 taattctcga aatggatgtc cttgtatttg tccagtatgg ctttgctgta tttgttcagt 1201 tcaatcgtca ggctgtcggc agtcttgact gtggttattt cgatgtggtc ggacttcaca 1261 tcgcttcttt tcagattgcg aacatccgaa taccgcaaac tcgtaaagca gcagaacagg 1321 aaaacatcac gcacacgttc caggtattgc ttatccttgg gtatctggta gtctttcagc 1381 ttgttcagtt catcccaagt caggaagatt acttttttcg aggtggtttt cagtttcggt 1441 ttgaacgtat cgtatgcaat gttctgatga tgtcctttct tgaagctcca gcgcaggaac 1501 catttgagga atcccatttg cttgccgatg gtgctgtttc tcatatcctt ggtgtcacgc 1561 aggaagttga cgtattcgtt caatccaaac tcgttgaaat agttgaacgt tgcatcctcc 1621 ttgaactctt tgaggtggtt cctcactgct gcaaattttt cataggtgga tgccgtccag 1681 ttattctggt taccgcactc ttttacaaac tcatcgaaca cctcccaaaa gctgacaggg 1741 gcttcttccg gctgttcttc gctggtgtct ttcattctca tgttgaaagc ttccttcaac 1801 tgttgggtcg ttggcatgac ctcctgcacc tcaaattcct tgaaaatatt ctggatttcg 1861 gcatagtatt tcagcaagtc cgtattgatt tcggctgcac tttgctttag cttgttggta 1921 catccgctct ttacccgctg cttatctgca tcccatttgg ctacgtcaat ccggtagccc 1981 gttgtaaact cgatgcgttg gctggcaaag atgacacgca tacggatggg tacgttctct 2041 acgattggca caccgttctt tttccggctc tccaatgcaa aaatgatgtt gcgcttgata 2101 ttcataattg ggtgcgtttg aaattctaca cccaaatata cacccaatta ttgagatagc 2161 aaaagacatt tagaaacatt tacttttact ctatattgta atttacactt gattatcagt 2221 cgtttgcagt cttatgatat tctgtgaaag tataagttcg agagcctgtc tctccgcaaa 2281 aaacgctgaa aatcagcaga ttgcaaaaca aacaccctgt tttacaccca agaatgtaaa 2341 gtcggctgtt tttgttttat ttaagataat acaaccacta cataataaaa gagtagcgat 2401 attaaaagaa tccgatgaga aaagactaat atttatctat ccattcagtt tgatttttca 2461 ggactttaca tcgtcctgaa agtatttgtt ggtaccggta ccgaggacgc gtaaacattt 2521 acagttgcat gtggcctatt gtttttagcc gttaaatatt ttataactat taaatagcga 2581 tacaaattgt tcgaaactaa tattgtttat atcatatatt ctcgcatgtt ttaaagcttt 2641 attaaattga ttttttgtaa acagtttttc gtactctttg ttaacccatt tcattacaaa 2701 agtttcatat ttttttctct ctttaaatgc catttttgct ggctttcttt ttaatacaat 2761 taatgtgcta tccactttag gttttggatg gaaataatac ctaggaattt ttgctaatat 2821 agaaatatct acctctgcca ttaacagcaa tgctagtgat ctgtttgtat ctaataacat 2881 tttagcaaaa ccatattcca ctattaaata acttattgtg gctgaacttt caaaaacaat 2941 ttttcgaatt atatttgtgc ttatgttgta aggtatgctg ccaaatattt tatatggatt 3001 gtggctagga aatgtaaatt tcagtatatc atcatttact atttgatagt taggataatt 3061 taagagctta ttacgagtta cctcacataa tttagaatca atttctatcg ccgttacaaa 3121 attacatctc tttaccaatc cagcagtaaa atgacctttc cctgcaccta tttcaaagat 3181 gttatctttt tcatctaaac ttatgcaatt cattattttt tctatgtgat attttgaagt 3241 aataaaattt tgactatctt ttatatttac tttgttcatt ataacctctc cttaatttat 3301 tgcatctctt ttcgaatatt tatgtttttt gagaaaagaa cgtactcatg gttcatcccg 3361 atatgcgtat cggtctgtat atcagcaact ttctatgtgt ttcaactaca atagtcatct 3421 attctcatct ttctgagtcc accccctgca aagcccctct ttacgacata aaaattcggt 3481 cggaaaaggt atgcaaaaga tgtttctctc tttaagagaa actcttcggg atgcaaaaat 3541 atgaaaataa ctccaattca ccaaattata tagcgacttt tttacaaaat gctaaaattt 3601 gttgatttcc gtcaagcaat tgttgagcaa aaatgtcttt tacgataaaa tgatacctca 3661 atatcaactg tttagcaaaa cgatatttct cttaaagaga gaaacacctt tttgttcacc 3721 aatccccgac ttttaatccc gcggccatga ttgaaaaagg aagagtatga gtattcaaca 3781 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 3841 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 3901 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 3961 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 4021 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 4081 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 4141 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 4201 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 4261 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 4321 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 4381 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 4441 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 4501 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 4561 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 4621 ttggtaactg tcagaccaag tttactcata acgcgtcaat tcgaggggga tcaattccgt 4681 gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc 4741 tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag 4801 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat 4861 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa 4921 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa 4981 gcagggttat gcagcggaaa acggaattga tccggccacg atgcgtccgg cgtagaggat 5041 ctgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact 5101 aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa 5161 cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat 5221 gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt 5281 tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac 5341 aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc aattttgagt 5401 gacacaggaa cacttaacgg ctgacatggg aattcccctc caccgcggtg gcggccgctg 5461 atctggaaga agcaatgaaa gctgctgtta agtctccgaa tcaggtattg ttcctgacag 5521 gtgtattccc atccggtaaa cgcggatact ttgcagttga tctgactcag gaataaatta 5581 taaattaagg taagaagatt gtaggataag ctaatgaaat agaaaaagga tgccgtcaca 5641 caacttgtcg gcattctttt ttgttttatt agttgaaaat atagtgaaaa agttgcctaa 5701 atatgtatgt taacaaatta tttgtcgtaa ctttgcactc caaatctgtt tttaacatat 5761 ggcacta //