LOCUS Exported 803 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION mammalian expression of ER localized oxBFP. ACCESSION . VERSION . KEYWORDS ERoxBFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 803) AUTHORS Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha VV, Snapp EL TITLE A palette of fluorescent proteins optimized for diverse cellular environments. JOURNAL Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. PUBMED 26158227 REFERENCE 2 (bases 1 to 803) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..803 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 10..19 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS complement(29..121) /codon_start=1 /product="signal sequence from bovine prolactin" /label=prolactin signal sequence /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVST" 5'UTR complement(130..172) /label=Xenopus globin 5'-UTR /note="translational enhancer from the 5'-UTR of the major beta-globin gene of Xenopus laevis" promoter complement(200..403) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(229..249) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 404..707 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 ccttgctcac catggtggcg accggtccgg tggagaccac accctggcac aagagtagat 61 ttgacaccac cagcagcagg agcaggcggg accctttctg cgacgaacct ttgctgtcca 121 tggtatctgc caaagttgag cgtttattct gagcttctgc aaaaagaaca agctagcgga 181 tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc 241 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt 301 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa 361 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga 421 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat 481 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca 541 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga 601 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt 661 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat 721 atgggctatg aactaatgac cccgtaattg attactatta ataactaatg catggcggta 781 atacggttat ccacagaatc agg //