LOCUS Exported 3510 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR223_BFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3510) AUTHORS Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE TITLE A public genome-scale lentiviral expression library of human ORFs. JOURNAL Nat Methods. 2011 Jun 26. ():. PUBMED 21706014 REFERENCE 2 (bases 1 to 3510) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3510 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 671..1387 /codon_start=1 /product="blue variant of GFP" /label=BFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(716..737) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(977..996) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1324..1345 /label=EGFP-C /note="EGFP, forward primer" protein_bind complement(1389..1488) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1505..1524) /label=T7 /note="T7 promoter, forward primer" promoter complement(1506..1524) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1529..1545) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1529..1545) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1976..2767 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2860..3448 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3349..3368 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agttggcacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 721 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 781 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 841 ctggcccacc ctcgtgacca ccctgagcca cggcgtccag tgcttcagcc gctaccccga 901 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 961 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 1021 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 1081 cctggggcac aagctggagt acaacttcaa cagccacaac gtctatatca tggccgacaa 1141 gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt 1201 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 1261 cgacagccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 1321 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 1381 gtacaagttg ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat 1441 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga 1501 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt 1561 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta 1621 gaccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac 1681 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct 1741 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg 1801 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc 1861 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg 1921 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag 1981 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg 2041 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct 2101 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac 2161 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat 2221 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc 2281 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt 2341 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag 2401 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct 2461 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga 2521 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat 2581 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc 2641 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg 2701 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg 2761 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca 2821 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 2881 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 2941 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 3001 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 3061 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 3121 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 3181 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 3241 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 3301 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 3361 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 3421 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 3481 ggccttttgc tggccttttg ctcacatgtt //