LOCUS Exported 3477 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR223_HcRed SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3477) AUTHORS Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE TITLE A public genome-scale lentiviral expression library of human ORFs. JOURNAL Nat Methods. 2011 Jun 26. ():. PUBMED 21706014 REFERENCE 2 (bases 1 to 3477) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3477 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 671..1354 /codon_start=1 /product="far-red fluorescent variant of Heteractis crispa chromoprotein" /label=HcRed1 /note="mammalian codon-optimized" /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD LPEKAN" protein_bind complement(1356..1455) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1472..1491) /label=T7 /note="T7 promoter, forward primer" promoter complement(1473..1491) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1496..1512) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1496..1512) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1943..2734 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2827..3415 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3316..3335 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agttggcacc atggtgagcg gcctgctgaa ggagagtatg cgcatcaaga tgtacatgga 721 gggcaccgtg aacggccact acttcaagtg cgagggcgag ggcgacggca accccttcgc 781 cggcacccag agcatgagaa tccacgtgac cgagggcgcc cccctgccct tcgccttcga 841 catcctggcc ccctgctgcg agtacggcag caggaccttc gtgcaccaca ccgccgagat 901 ccccgacttc ttcaagcaga gcttccccga gggcttcacc tgggagagaa ccaccaccta 961 cgaggacggc ggcatcctga ccgcccacca ggacaccagc ctggagggca actgcctgat 1021 ctacaaggtg aaggtgcacg gcaccaactt ccccgccgac ggccccgtga tgaagaacaa 1081 gagcggcggc tgggagccca gcaccgaggt ggtgtacccc gagaacggcg tgctgtgcgg 1141 ccggaacgtg atggccctga aggtgggcga ccggcacctg atctgccacc actacaccag 1201 ctaccggagc aagaaggccg tgcgcgccct gaccatgccc ggcttccact tcaccgacat 1261 ccggctccag atgctgcgga agaagaagga cgagtacttc gagctgtacg aggccagcgt 1321 ggcccggtac agcgacctgc ccgagaaggc caacttgcca actttcttgt acaaagttgg 1381 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa 1441 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat 1501 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga 1561 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct 1621 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg 1681 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg 1741 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg 1801 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta 1861 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg 1921 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact 1981 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt 2041 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac 2101 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac 2161 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca 2221 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca 2281 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat 2341 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact 2401 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg 2461 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat 2521 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat 2581 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg 2641 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt 2701 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc 2761 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 2821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 2881 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 2941 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 3001 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 3061 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 3121 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 3181 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc 3241 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 3301 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 3361 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 3421 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt //