LOCUS Exported 6521 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Rhamnose-inducible Int12 expression vector with rpiL*C51 RBS, pNBU2 backbone, AmpR. ACCESSION . VERSION . KEYWORDS pAT937 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6521) AUTHORS Mimee M, Tucker AC, Voigt CA, Lu TK TITLE Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. JOURNAL Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001. PUBMED 26918244 REFERENCE 2 (bases 1 to 6521) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6521 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 3022..3882 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3240..3259) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" oriT 4013..4122 /note="incP origin of transfer" rep_origin 4293..4681 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" ORIGIN 1 ccttgttgct catattgtga tgataatttc tacaaatata gtcattggta actatctatg 61 aaactgtttg atacttttat agttgattaa acttgttcat ggcatttgcc ttaatatcat 121 ccgctatgtc aatgtagggt ttcatagctt tgtagtcgct gtgtcccgtc catttcatga 181 ccacctgtgc cgggattccg agagccagcg cattgcagat gaatgtcctt cttcctgcat 241 gggtactgag caaagcgtat ttgggtgtga cttcatcaat acgttcattt cccttgtagt 301 aggtttcccg tacaggctcg ttgatttctg ccagttcgcc cagctctttc aggtaatcgt 361 tcatcttctg gttgctgatg acgggcagag ccatgtaatt ctcgaaatgg atgtccttgt 421 atttgtccag tatggctttg ctgtatttgt tcagttcaat cgtcaggctg tcggcagtct 481 tgactgtggt tatttcgatg tggtcggact tcacatcgct tcttttcaga ttgcgaacat 541 ccgaataccg caaactcgta aagcagcaga acaggaaaac atcacgcaca cgttccaggt 601 attgcttatc cttgggtatc tggtagtctt tcagcttgtt cagttcatcc caagtcagga 661 agattacttt tttcgaggtg gttttcagtt tcggtttgaa cgtatcgtat gcaatgttct 721 gatgatgtcc tttcttgaag ctccagcgca ggaaccattt gaggaatccc atttgcttgc 781 cgatggtgct gtttctcata tccttggtgt cacgcaggaa gttgacgtat tcgttcaatc 841 caaactcgtt gaaatagttg aacgttgcat cctccttgaa ctctttgagg tggttcctca 901 ctgctgcaaa tttttcatag gtggatgccg tccagttatt ctggttaccg cactctttta 961 caaactcatc gaacacctcc caaaagctga caggggcttc ttccggctgt tcttcgctgg 1021 tgtctttcat tctcatgttg aaagcttcct tcaactgttg ggtcgttggc atgacctcct 1081 gcacctcaaa ttccttgaaa atattctgga tttcggcata gtatttcagc aagtccgtat 1141 tgatttcggc tgcactttgc tttagcttgt tggtacatcc gctctttacc cgctgcttat 1201 ctgcatccca tttggctacg tcaatccggt agcccgttgt aaactcgatg cgttggctgg 1261 caaagatgac acgcatacgg atgggtacgt tctctacgat tggcacaccg ttctttttcc 1321 ggctctccaa tgcaaaaatg atgttgcgct tgatattcat aattgggtgc gtttgaaatt 1381 ctacacccaa atatacaccc aattattgag atagcaaaag acatttagaa acatttactt 1441 ttactctata ttgtaattta cacttgatta tcagtcgttt gcagtcttat gatattctgt 1501 gaaagtataa gttcgagagc ctgtctctcc gcaaaaaacg ctgaaaatca gcagattgca 1561 aaacaaacac cctgttttac acccaagaat gtaaagtcgg ctgtttttgt tttatttaag 1621 ataatacaac cactacataa taaaagagta gcgatattaa aagaatccga tgagaaaaga 1681 ctaatattta tctatccatt cagtttgatt tttcaggact ttacatcgtc ctgaaagtat 1741 ttgttggtac cggtaccgag gacgcgtaaa catttacagt tgcatgtggc ctattgtttt 1801 tagccgttaa atattttata actattaaat agcgatacaa attgttcgaa actaatattg 1861 tttatatcat atattctcgc atgttttaaa gctttattaa attgattttt tgtaaacagt 1921 ttttcgtact ctttgttaac ccatttcatt acaaaagttt catatttttt tctctcttta 1981 aatgccattt ttgctggctt tctttttaat acaattaatg tgctatccac tttaggtttt 2041 ggatggaaat aatacctagg aatttttgct aatatagaaa tatctacctc tgccattaac 2101 agcaatgcta gtgatctgtt tgtatctaat aacattttag caaaaccata ttccactatt 2161 aaataactta ttgtggctga actttcaaaa acaatttttc gaattatatt tgtgcttatg 2221 ttgtaaggta tgctgccaaa tattttatat ggattgtggc taggaaatgt aaatttcagt 2281 atatcatcat ttactatttg atagttagga taatttaaga gcttattacg agttacctca 2341 cataatttag aatcaatttc tatcgccgtt acaaaattac atctctttac caatccagca 2401 gtaaaatgac ctttccctgc acctatttca aagatgttat ctttttcatc taaacttatg 2461 caattcatta ttttttctat gtgatatttt gaagtaataa aattttgact atcttttata 2521 tttactttgt tcattataac ctctccttaa tttattgcat ctcttttcga atatttatgt 2581 tttttgagaa aagaacgtac tcatggttca tcccgatatg cgtatcggtc tgtatatcag 2641 caactttcta tgtgtttcaa ctacaatagt catctattct catctttctg agtccacccc 2701 ctgcaaagcc cctctttacg acataaaaat tcggtcggaa aaggtatgca aaagatgttt 2761 ctctctttaa gagaaactct tcgggatgca aaaatatgaa aataactcca attcaccaaa 2821 ttatatagcg acttttttac aaaatgctaa aatttgttga tttccgtcaa gcaattgttg 2881 agcaaaaatg tcttttacga taaaatgata cctcaatatc aactgtttag caaaacgata 2941 tttctcttaa agagagaaac acctttttgt tcaccaatcc ccgactttta atcccgcggc 3001 catgattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 3061 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 3121 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 3181 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 3241 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 3301 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 3361 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 3421 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 3481 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 3541 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 3601 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 3661 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 3721 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 3781 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 3841 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 3901 tcataacgcg tcaattcgag ggggatcaat tccgtgatag gtgggctgcc cttcctggtt 3961 ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag 4021 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag 4081 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata 4141 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat 4201 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaacgga 4261 attgatccgg ccacgatgcg tccggcgtag aggatctgaa gatcagcagt tcaacctgtt 4321 gatagtacgt actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag 4381 ctctcatgtt taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt 4441 actaagctct catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa 4501 tcagcaactt aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag 4561 gcttttaaag cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg 4621 agaagccctt agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac 4681 atgggaattc ccctccaccg cggtggtatt tcggagaaaa catgcataaa tcatgctttt 4741 tttgcataaa aagtaaaatt tatactgatg taaggtttgg ctatgcagat ttgtgtcaaa 4801 atgcacatcc tttctatcaa aatgcgtaag gaaaaggagg aaggaaccgc ctatctttgc 4861 aatgtacgca ttttaaaata aaataaatta ttattttatt taaacgaata tgaaagtggc 4921 catttatacc cgtgttagca gcgcagaaca ggcaaatgaa ggttatagca ttcacgagca 4981 gaagaagaaa ctgatcagct attgcgaaat ccacgattgg aacgagtata aagtttttac 5041 cgatgcaggt attagcggtg gtagcatgaa acgtccggca ctgcaaaaac tgatgaaaca 5101 tctgagttca tttgatctgg tgctggtgta taaactggat cgtctgaccc gtaatgttcg 5161 tgatctgctg gatatgctgg aagaatttga acagtataac gtgagcttta aaagcgccac 5221 cgaagttttt gataccacca gtgcaattgg caaactgttt attaccatgg ttggtgcaat 5281 ggcagaatgg gaacgtgaaa ccattcgtga acgtagcctg tttggtagcc gtgcagcagt 5341 tcgtgaaggt aactatattc gtgaagcacc gttttgctat gataacattg aaggtaaact 5401 gcacccgaac gaatatgcca aagttattga tctgattgtg agcatgttca aaaaaggcat 5461 tagcgccaat gaaattgcac gtcgtctgaa tagcagcaaa gttcatgttc cgaacaaaaa 5521 aagctggaat cgtaatagcc tgattcgtct gatgcgtagt ccggttctgc gtggtcatac 5581 caaatatggt gatatgctga ttgaaaacac ccatgaaccg gtgctgagcg aacatgatta 5641 taatgcaatt aacaacgcca tcagcagcaa aacccataaa agcaaagtta aacaccatgc 5701 catttttcgt ggtgcactgg tttgtccgca gtgtaatcgt cgtctgcatc tgtatgcagg 5761 caccgttaaa gatcgtaaag gctataaata cgatgtgcgt cgctataaat gtgaaacctg 5821 cagcaaaaac aaagatgtga agaatgtgag cttcaacgaa agcgaagtgg aaaacaaatt 5881 cgtcaatctg ctgaaaagct acgagctgaa caaatttcat atccgtaaag tggaaccggt 5941 gaaaaaaatc gagtatgaca tcgataagat taacaaacag aaaattaact atacccgcag 6001 ttggagcctg ggctatattg aagatgatga atatttcgag ctgatggaag aaatcaacgc 6061 caccaaaaaa atgatcgaag aacagaccac cgagaataaa cagagcgtta gcaaagagca 6121 gattcagagc attaacaact ttatcctgaa aggctgggaa gaactgacca tcaaagataa 6181 agaggaactg attctgagca ccgtggataa aatcgaattt aacttcatcc cgaaagataa 6241 aaaacataaa accaataccc tggatattaa caatattcac tttaaattct aatgaactgc 6301 acttgctttg ataattaatg ataaacaatc taaaagcact ctaatcgtta tcggagtgct 6361 tttagattac taatcaaatt gcttctacta attgcctatc ttccagtgat ggaacagcat 6421 ttgtgcattg gctgcaacaa tcagccttac ttgtgcctgt tctatttccg aaccgaccgc 6481 ttgtatgaat ccatcaaaat tcgttttctc tatgttggat t //