LOCUS       Exported                5784 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  NanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 
            backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    pAT593
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3037..3897
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3255..3274)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4028..4137
                     /note="incP origin of transfer"
     rep_origin      4308..4696
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 tctctatgtt ggattccttg ttgctcatat tgtgatgata atttctacaa atatagtcat
       61 tggtaactat ctatgaaact gtttgatact tttatagttg attaaacttg ttcatggcat
      121 ttgccttaat atcatccgct atgtcaatgt agggtttcat agctttgtag tcgctgtgtc
      181 ccgtccattt catgaccacc tgtgccggga ttccgagagc cagcgcattg cagatgaatg
      241 tccttcttcc tgcatgggta ctgagcaaag cgtatttggg tgtgacttca tcaatacgtt
      301 catttccctt gtagtaggtt tcccgtacag gctcgttgat ttctgccagt tcgcccagct
      361 ctttcaggta atcgttcatc ttctggttgc tgatgacggg cagagccatg taattctcga
      421 aatggatgtc cttgtatttg tccagtatgg ctttgctgta tttgttcagt tcaatcgtca
      481 ggctgtcggc agtcttgact gtggttattt cgatgtggtc ggacttcaca tcgcttcttt
      541 tcagattgcg aacatccgaa taccgcaaac tcgtaaagca gcagaacagg aaaacatcac
      601 gcacacgttc caggtattgc ttatccttgg gtatctggta gtctttcagc ttgttcagtt
      661 catcccaagt caggaagatt acttttttcg aggtggtttt cagtttcggt ttgaacgtat
      721 cgtatgcaat gttctgatga tgtcctttct tgaagctcca gcgcaggaac catttgagga
      781 atcccatttg cttgccgatg gtgctgtttc tcatatcctt ggtgtcacgc aggaagttga
      841 cgtattcgtt caatccaaac tcgttgaaat agttgaacgt tgcatcctcc ttgaactctt
      901 tgaggtggtt cctcactgct gcaaattttt cataggtgga tgccgtccag ttattctggt
      961 taccgcactc ttttacaaac tcatcgaaca cctcccaaaa gctgacaggg gcttcttccg
     1021 gctgttcttc gctggtgtct ttcattctca tgttgaaagc ttccttcaac tgttgggtcg
     1081 ttggcatgac ctcctgcacc tcaaattcct tgaaaatatt ctggatttcg gcatagtatt
     1141 tcagcaagtc cgtattgatt tcggctgcac tttgctttag cttgttggta catccgctct
     1201 ttacccgctg cttatctgca tcccatttgg ctacgtcaat ccggtagccc gttgtaaact
     1261 cgatgcgttg gctggcaaag atgacacgca tacggatggg tacgttctct acgattggca
     1321 caccgttctt tttccggctc tccaatgcaa aaatgatgtt gcgcttgata ttcataattg
     1381 ggtgcgtttg aaattctaca cccaaatata cacccaatta ttgagatagc aaaagacatt
     1441 tagaaacatt tacttttact ctatattgta atttacactt gattatcagt cgtttgcagt
     1501 cttatgatat tctgtgaaag tataagttcg agagcctgtc tctccgcaaa aaacgctgaa
     1561 aatcagcaga ttgcaaaaca aacaccctgt tttacaccca agaatgtaaa gtcggctgtt
     1621 tttgttttat ttaagataat acaaccacta cataataaaa gagtagcgat attaaaagaa
     1681 tccgatgaga aaagactaat atttatctat ccattcagtt tgatttttca ggactttaca
     1741 tcgtcctgaa agtatttgtt ggtaccggta ccgaggacgc gtaaacattt acagttgcat
     1801 gtggcctatt gtttttagcc gttaaatatt ttataactat taaatagcga tacaaattgt
     1861 tcgaaactaa tattgtttat atcatatatt ctcgcatgtt ttaaagcttt attaaattga
     1921 ttttttgtaa acagtttttc gtactctttg ttaacccatt tcattacaaa agtttcatat
     1981 ttttttctct ctttaaatgc catttttgct ggctttcttt ttaatacaat taatgtgcta
     2041 tccactttag gttttggatg gaaataatac ctaggaattt ttgctaatat agaaatatct
     2101 acctctgcca ttaacagcaa tgctagtgat ctgtttgtat ctaataacat tttagcaaaa
     2161 ccatattcca ctattaaata acttattgtg gctgaacttt caaaaacaat ttttcgaatt
     2221 atatttgtgc ttatgttgta aggtatgctg ccaaatattt tatatggatt gtggctagga
     2281 aatgtaaatt tcagtatatc atcatttact atttgatagt taggataatt taagagctta
     2341 ttacgagtta cctcacataa tttagaatca atttctatcg ccgttacaaa attacatctc
     2401 tttaccaatc cagcagtaaa atgacctttc cctgcaccta tttcaaagat gttatctttt
     2461 tcatctaaac ttatgcaatt cattattttt tctatgtgat attttgaagt aataaaattt
     2521 tgactatctt ttatatttac tttgttcatt ataacctctc cttaatttat tgcatctctt
     2581 ttcgaatatt tatgtttttt gagaaaagaa cgtactcatg gttcatcccg atatgcgtat
     2641 cggtctgtat atcagcaact ttctatgtgt ttcaactaca atagtcatct attctcatct
     2701 ttctgagtcc accccctgca aagcccctct ttacgacata aaaattcggt cggaaaaggt
     2761 atgcaaaaga tgtttctctc tttaagagaa actcttcggg atgcaaaaat atgaaaataa
     2821 ctccaattca ccaaattata tagcgacttt tttacaaaat gctaaaattt gttgatttcc
     2881 gtcaagcaat tgttgagcaa aaatgtcttt tacgataaaa tgatacctca atatcaactg
     2941 tttagcaaaa cgatatttct cttaaagaga gaaacacctt tttgttcacc aatccccgac
     3001 ttttaatccc gcggccatga ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3061 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3121 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3181 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3241 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3301 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3361 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3421 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3481 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3541 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3601 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3661 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3721 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3781 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3841 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3901 tcagaccaag tttactcata acgcgtcaat tcgaggggga tcaattccgt gataggtggg
     3961 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
     4021 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
     4081 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
     4141 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
     4201 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
     4261 gcagcggaaa acggaattga tccggccacg atgcgtccgg cgtagaggat ctgaagatca
     4321 gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact aagctctcat
     4381 gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc
     4441 tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat gtttgaacaa
     4501 taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt tataagaaaa
     4561 aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac aacaagccag
     4621 ggatgtaacg cactgagaag cccttagagc ctctcaaagc aattttgagt gacacaggaa
     4681 cacttaacgg ctgacatggg aattcccctc caccgcggtg gcggccgctg atctggaaga
     4741 agcaatgaaa gctgctgtta agtctccgaa tcaggtattg ttcctgacag gtgtattccc
     4801 atccggtaaa cgcggatact ttgcagttga tctgactcag gaataaatta taaattaagg
     4861 taagaagatt gtaggataag ctaatgaaat agaaaaagga tgccgtcaca caacttgtcg
     4921 gcattctttt ttgttttatt agttgaaaat atagtgaaaa agttgcctaa atatgtatgt
     4981 taacaaatta tttgtcgtaa ctttgcactc ccgcatttta aaataaaata aattatttat
     5041 ttaattaaac gaatatggtt tttactctgg aagattttgt tggcgattgg cgtcagaccg
     5101 cgggttataa tttggatcaa gtcctggaac agggtggcgt aagctctctg ttccagaacc
     5161 tgggtgtgag cgtgacgccg attcagcgca tcgttctgtc cggcgagaac ggtctgaaaa
     5221 ttgatattca tgtgatcatc ccgtacgaag gcctgagcgg tgaccaaatg ggtcaaatcg
     5281 agaaaatctt taaagtcgtc tacccagttg acgatcacca cttcaaggtt atcttgcatt
     5341 acggtacgct ggtgattgat ggtgtgaccc cgaatatgat tgactatttc ggccgtccgt
     5401 atgaaggcat tgccgttttt gacggtaaaa agatcaccgt caccggtacc ctgtggaatg
     5461 gcaataagat tattgacgag cgtctgatta acccggacgg cagcctgctg ttccgcgtga
     5521 ccatcaacgg tgtcacgggt tggcgtctgt gcgagcgcat cctggcataa tgaactgcac
     5581 ttgctttgat aattaatgat aaacaatcta aaagcactct aatcgttatc ggagtgcttt
     5641 tagattacta atcaaattgc ttctactaat tgcctatctt ccagtgatgg aacagcattt
     5701 gtgcattggc tgcaacaatc agccttactt gtgcctgttc tatttccgaa ccgaccgctt
     5761 gtatgaatcc atcaaaattc gttt
//
