LOCUS Exported 5706 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Arabinogalactan-inducible NanoLuc expression, pNBU2 backbone, AmpR. ACCESSION . VERSION . KEYWORDS pMM660 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5706) AUTHORS Mimee M, Tucker AC, Voigt CA, Lu TK TITLE Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. JOURNAL Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001. PUBMED 26918244 REFERENCE 2 (bases 1 to 5706) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5706 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 3743..4603 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3961..3980) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" oriT 4734..4843 /note="incP origin of transfer" rep_origin 5014..5402 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" ORIGIN 1 gttggcgatt ggcgtcagac cgcgggttat aatttggatc aagtcctgga acagggtggc 61 gtaagctctc tgttccagaa cctgggtgtg agcgtgacgc cgattcagcg catcgttctg 121 tccggcgaga acggtctgaa aattgatatt catgtgatca tcccgtacga aggcctgagc 181 ggtgaccaaa tgggtcaaat cgagaaaatc tttaaagtcg tctacccagt tgacgatcac 241 cacttcaagg ttatcttgca ttacggtacg ctggtgattg atggtgtgac cccgaatatg 301 attgactatt tcggccgtcc gtatgaaggc attgccgttt ttgacggtaa aaagatcacc 361 gtcaccggta ccctgtggaa tggcaataag attattgacg agcgtctgat taacccggac 421 ggcagcctgc tgttccgcgt gaccatcaac ggtgtcacgg gttggcgtct gtgcgagcgc 481 atcctggcat aatgaactgc acttgctttg ataattaatg ataaacaatc taaaagcact 541 ctaatcgtta tcggagtgct tttagattac taatcaaatt gcttctacta attgcctatc 601 ttccagtgat ggaacagcat ttgtgcattg gctgcaacaa tcagccttac ttgtgcctgt 661 tctatttccg aaccgaccgc ttgtatgaat ccatcaaaat tcgttttctc tatgttggat 721 tccttgttgc tcatattgtg atgataattt ctacaaatat agtcattggt aactatctat 781 gaaactgttt gatactttta tagttgatta aacttgttca tggcatttgc cttaatatca 841 tccgctatgt caatgtaggg tttcatagct ttgtagtcgc tgtgtcccgt ccatttcatg 901 accacctgtg ccgggattcc gagagccagc gcattgcaga tgaatgtcct tcttcctgca 961 tgggtactga gcaaagcgta tttgggtgtg acttcatcaa tacgttcatt tcccttgtag 1021 taggtttccc gtacaggctc gttgatttct gccagttcgc ccagctcttt caggtaatcg 1081 ttcatcttct ggttgctgat gacgggcaga gccatgtaat tctcgaaatg gatgtccttg 1141 tatttgtcca gtatggcttt gctgtatttg ttcagttcaa tcgtcaggct gtcggcagtc 1201 ttgactgtgg ttatttcgat gtggtcggac ttcacatcgc ttcttttcag attgcgaaca 1261 tccgaatacc gcaaactcgt aaagcagcag aacaggaaaa catcacgcac acgttccagg 1321 tattgcttat ccttgggtat ctggtagtct ttcagcttgt tcagttcatc ccaagtcagg 1381 aagattactt ttttcgaggt ggttttcagt ttcggtttga acgtatcgta tgcaatgttc 1441 tgatgatgtc ctttcttgaa gctccagcgc aggaaccatt tgaggaatcc catttgcttg 1501 ccgatggtgc tgtttctcat atccttggtg tcacgcagga agttgacgta ttcgttcaat 1561 ccaaactcgt tgaaatagtt gaacgttgca tcctccttga actctttgag gtggttcctc 1621 actgctgcaa atttttcata ggtggatgcc gtccagttat tctggttacc gcactctttt 1681 acaaactcat cgaacacctc ccaaaagctg acaggggctt cttccggctg ttcttcgctg 1741 gtgtctttca ttctcatgtt gaaagcttcc ttcaactgtt gggtcgttgg catgacctcc 1801 tgcacctcaa attccttgaa aatattctgg atttcggcat agtatttcag caagtccgta 1861 ttgatttcgg ctgcactttg ctttagcttg ttggtacatc cgctctttac ccgctgctta 1921 tctgcatccc atttggctac gtcaatccgg tagcccgttg taaactcgat gcgttggctg 1981 gcaaagatga cacgcatacg gatgggtacg ttctctacga ttggcacacc gttctttttc 2041 cggctctcca atgcaaaaat gatgttgcgc ttgatattca taattgggtg cgtttgaaat 2101 tctacaccca aatatacacc caattattga gatagcaaaa gacatttaga aacatttact 2161 tttactctat attgtaattt acacttgatt atcagtcgtt tgcagtctta tgatattctg 2221 tgaaagtata agttcgagag cctgtctctc cgcaaaaaac gctgaaaatc agcagattgc 2281 aaaacaaaca ccctgtttta cacccaagaa tgtaaagtcg gctgtttttg ttttatttaa 2341 gataatacaa ccactacata ataaaagagt agcgatatta aaagaatccg atgagaaaag 2401 actaatattt atctatccat tcagtttgat ttttcaggac tttacatcgt cctgaaagta 2461 tttgttggta ccggtaccga ggacgcgtaa acatttacag ttgcatgtgg cctattgttt 2521 ttagccgtta aatattttat aactattaaa tagcgataca aattgttcga aactaatatt 2581 gtttatatca tatattctcg catgttttaa agctttatta aattgatttt ttgtaaacag 2641 tttttcgtac tctttgttaa cccatttcat tacaaaagtt tcatattttt ttctctcttt 2701 aaatgccatt tttgctggct ttctttttaa tacaattaat gtgctatcca ctttaggttt 2761 tggatggaaa taatacctag gaatttttgc taatatagaa atatctacct ctgccattaa 2821 cagcaatgct agtgatctgt ttgtatctaa taacatttta gcaaaaccat attccactat 2881 taaataactt attgtggctg aactttcaaa aacaattttt cgaattatat ttgtgcttat 2941 gttgtaaggt atgctgccaa atattttata tggattgtgg ctaggaaatg taaatttcag 3001 tatatcatca tttactattt gatagttagg ataatttaag agcttattac gagttacctc 3061 acataattta gaatcaattt ctatcgccgt tacaaaatta catctcttta ccaatccagc 3121 agtaaaatga cctttccctg cacctatttc aaagatgtta tctttttcat ctaaacttat 3181 gcaattcatt attttttcta tgtgatattt tgaagtaata aaattttgac tatcttttat 3241 atttactttg ttcattataa cctctcctta atttattgca tctcttttcg aatatttatg 3301 ttttttgaga aaagaacgta ctcatggttc atcccgatat gcgtatcggt ctgtatatca 3361 gcaactttct atgtgtttca actacaatag tcatctattc tcatctttct gagtccaccc 3421 cctgcaaagc ccctctttac gacataaaaa ttcggtcgga aaaggtatgc aaaagatgtt 3481 tctctcttta agagaaactc ttcgggatgc aaaaatatga aaataactcc aattcaccaa 3541 attatatagc gactttttta caaaatgcta aaatttgttg atttccgtca agcaattgtt 3601 gagcaaaaat gtcttttacg ataaaatgat acctcaatat caactgttta gcaaaacgat 3661 atttctctta aagagagaaa cacctttttg ttcaccaatc cccgactttt aatcccgcgg 3721 ccatgattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 3781 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 3841 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 3901 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 3961 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 4021 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 4081 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 4141 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 4201 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 4261 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 4321 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 4381 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 4441 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 4501 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 4561 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 4621 ctcataacgc gtcaattcga gggggatcaa ttccgtgata ggtgggctgc ccttcctggt 4681 tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca 4741 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa 4801 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat 4861 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga 4921 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaacgg 4981 aattgatccg gccacgatgc gtccggcgta gaggatctga agatcagcag ttcaacctgt 5041 tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa 5101 gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg 5161 tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa 5221 atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa 5281 ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact 5341 gagaagccct tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga 5401 catgggaatt cccctccacc gcggtggtaa aatacacaag tacgcgtctt aatggaagat 5461 gcgtactttt ccatatatca atgatctatc ccatttgaat gattcctgaa cttatattga 5521 acgattttta gacctgttat agttaatagc gattatggtc caattttgga agtttttgaa 5581 tgattagaga acttctttct actggataac tcgcactttt gtgacgcatt tgatgcacaa 5641 ctaatactta tttggtctaa ataactttat aaatctaata gtatggtttt tactctggaa 5701 gatttt //