LOCUS       Exported               11677 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    pMM732
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             326..1408
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(345..364)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             4571..5431
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4789..4808)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            5562..5671
                     /note="incP origin of transfer"
     rep_origin      5842..6230
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    7163..7179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    7196..7212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             7257..11363
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        11523..11598
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tcagccttga tctggaagaa gcaatgaaag ctgctgttaa gtctccgaat caggtattgt
       61 tcctgacagg tgtattccca tccggtaaac gcggatactt tgcagttgat ctgactcagg
      121 aataaattat aaattaaggt aagaagattg taggataagc taatgaaata gaaaaaggat
      181 gccgtcacac aacttgtcgg cattcttttt tgttttatta gttgaaaata tagtgaaaaa
      241 gttgcctaaa tatgtatgtt aacaaattat ttgtcgtaac tttgcactcc aaatctgttt
      301 ttaacatatg gcactagtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
      361 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
      421 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
      481 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
      541 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
      601 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
      661 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
      721 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
      781 ccagacaccc atcaacagta ttattttctc ccatgaggac ggtacgcgac tgggcgtgga
      841 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
      901 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
      961 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
     1021 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
     1081 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
     1141 atacgacgat accgaggaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
     1201 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     1261 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     1321 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     1381 ggtttcccga ctggaaagcg ggcagtgagc tttcctcggt accaaattcc agaaaagagg
     1441 cctcccgaaa ggggggcctt ttttcgtttt ggtcctactt gtgcctgttc tatttccgaa
     1501 ccgaccgctt gtatgaatcc atcaaaattc gttttctcta tgttggattc cttgttgctc
     1561 atattgtgat gataatttct acaaatatag tcattggtaa ctatctatga aactgtttga
     1621 tacttttata gttgattaaa cttgttcatg gcatttgcct taatatcatc cgctatgtca
     1681 atgtagggtt tcatagcttt gtagtcgctg tgtcccgtcc atttcatgac cacctgtgcc
     1741 gggattccga gagccagcgc attgcagatg aatgtccttc ttcctgcatg ggtactgagc
     1801 aaagcgtatt tgggtgtgac ttcatcaata cgttcatttc ccttgtagta ggtttcccgt
     1861 acaggctcgt tgatttctgc cagttcgccc agctctttca ggtaatcgtt catcttctgg
     1921 ttgctgatga cgggcagagc catgtaattc tcgaaatgga tgtccttgta tttgtccagt
     1981 atggctttgc tgtatttgtt cagttcaatc gtcaggctgt cggcagtctt gactgtggtt
     2041 atttcgatgt ggtcggactt cacatcgctt cttttcagat tgcgaacatc cgaataccgc
     2101 aaactcgtaa agcagcagaa caggaaaaca tcacgcacac gttccaggta ttgcttatcc
     2161 ttgggtatct ggtagtcttt cagcttgttc agttcatccc aagtcaggaa gattactttt
     2221 ttcgaggtgg ttttcagttt cggtttgaac gtatcgtatg caatgttctg atgatgtcct
     2281 ttcttgaagc tccagcgcag gaaccatttg aggaatccca tttgcttgcc gatggtgctg
     2341 tttctcatat ccttggtgtc acgcaggaag ttgacgtatt cgttcaatcc aaactcgttg
     2401 aaatagttga acgttgcatc ctccttgaac tctttgaggt ggttcctcac tgctgcaaat
     2461 ttttcatagg tggatgccgt ccagttattc tggttaccgc actcttttac aaactcatcg
     2521 aacacctccc aaaagctgac aggggcttct tccggctgtt cttcgctggt gtctttcatt
     2581 ctcatgttga aagcttcctt caactgttgg gtcgttggca tgacctcctg cacctcaaat
     2641 tccttgaaaa tattctggat ttcggcatag tatttcagca agtccgtatt gatttcggct
     2701 gcactttgct ttagcttgtt ggtacatccg ctctttaccc gctgcttatc tgcatcccat
     2761 ttggctacgt caatccggta gcccgttgta aactcgatgc gttggctggc aaagatgaca
     2821 cgcatacgga tgggtacgtt ctctacgatt ggcacaccgt tctttttccg gctctccaat
     2881 gcaaaaatga tgttgcgctt gatattcata attgggtgcg tttgaaattc tacacccaaa
     2941 tatacaccca attattgaga tagcaaaaga catttagaaa catttacttt tactctatat
     3001 tgtaatttac acttgattat cagtcgtttg cagtcttatg atattctgtg aaagtataag
     3061 ttcgagagcc tgtctctccg caaaaaacgc tgaaaatcag cagattgcaa aacaaacacc
     3121 ctgttttaca cccaagaatg taaagtcggc tgtttttgtt ttatttaaga taatacaacc
     3181 actacataat aaaagagtag cgatattaaa agaatccgat gagaaaagac taatatttat
     3241 ctatccattc agtttgattt ttcaggactt tacatcgtcc tgaaagtatt tgttggtacc
     3301 ggtaccgagg acgcgtaaac atttacagtt gcatgtggcc tattgttttt agccgttaaa
     3361 tattttataa ctattaaata gcgatacaaa ttgttcgaaa ctaatattgt ttatatcata
     3421 tattctcgca tgttttaaag ctttattaaa ttgatttttt gtaaacagtt tttcgtactc
     3481 tttgttaacc catttcatta caaaagtttc atattttttt ctctctttaa atgccatttt
     3541 tgctggcttt ctttttaata caattaatgt gctatccact ttaggttttg gatggaaata
     3601 atacctagga atttttgcta atatagaaat atctacctct gccattaaca gcaatgctag
     3661 tgatctgttt gtatctaata acattttagc aaaaccatat tccactatta aataacttat
     3721 tgtggctgaa ctttcaaaaa caatttttcg aattatattt gtgcttatgt tgtaaggtat
     3781 gctgccaaat attttatatg gattgtggct aggaaatgta aatttcagta tatcatcatt
     3841 tactatttga tagttaggat aatttaagag cttattacga gttacctcac ataatttaga
     3901 atcaatttct atcgccgtta caaaattaca tctctttacc aatccagcag taaaatgacc
     3961 tttccctgca cctatttcaa agatgttatc tttttcatct aaacttatgc aattcattat
     4021 tttttctatg tgatattttg aagtaataaa attttgacta tcttttatat ttactttgtt
     4081 cattataacc tctccttaat ttattgcatc tcttttcgaa tatttatgtt ttttgagaaa
     4141 agaacgtact catggttcat cccgatatgc gtatcggtct gtatatcagc aactttctat
     4201 gtgtttcaac tacaatagtc atctattctc atctttctga gtccaccccc tgcaaagccc
     4261 ctctttacga cataaaaatt cggtcggaaa aggtatgcaa aagatgtttc tctctttaag
     4321 agaaactctt cgggatgcaa aaatatgaaa ataactccaa ttcaccaaat tatatagcga
     4381 cttttttaca aaatgctaaa atttgttgat ttccgtcaag caattgttga gcaaaaatgt
     4441 cttttacgat aaaatgatac ctcaatatca actgtttagc aaaacgatat ttctcttaaa
     4501 gagagaaaca cctttttgtt caccaatccc cgacttttaa tcccgcggcc atgattgaaa
     4561 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4621 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4681 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4741 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4801 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4861 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4921 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4981 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5041 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5101 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5161 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5221 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5281 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5341 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5401 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact cataacgcgt
     5461 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
     5521 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     5581 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     5641 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     5701 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     5761 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaacggaa ttgatccggc
     5821 cacgatgcgt ccggcgtaga ggatctgaag atcagcagtt caacctgttg atagtacgta
     5881 ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt
     5941 aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc
     6001 atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta
     6061 aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc
     6121 ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta
     6181 gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca tgggaattcc
     6241 cctccaccgc ggtgggttcc atcactggaa gataggcaat tagtagaagc aatttgatta
     6301 gtaatctaaa agcactccga taacgattag agtgctttta gattgtttat cattaattat
     6361 caaagcaagt gcagttcatt atgccaggat gcgctcgcac agacgccaac ccgtgacacc
     6421 gttgatggtc acgcggaaca gcaggctgcc gtccgggtta atcagacgct cgtcaataat
     6481 cttattgcca ttccacaggg taccggtgac ggtgatcttt ttaccgtcaa aaacggcaat
     6541 gccttcatac ggacggccga aatagtcaat catattcggg gtcacaccat caatcaccag
     6601 cgtaccgtaa tgcaagataa ccttgaagtg gtgatcgtca actgggtaga cgactttaaa
     6661 gattttctcg atttgaccca tttggtcacc gctcaggcct tcgtacggga tgatcacatg
     6721 aatatcaatt ttcagaccgt tctcgccgga cagaacgatg cgctgaatcg gcgtcacgct
     6781 cacacccagg ttctggaaca gagagcttac gccaccctgt tccaggactt gatccaaatt
     6841 ataacccgcg gtctgacgcc aatcgccaac aaaatcttcc agagtaaaaa ccattttatt
     6901 cttttacctt aattccgcaa cactattata aaatattttt tttaagtacc tgagcaacaa
     6961 aaagttgctc aggattttgc catgtcagag gattcactta acttagtgtt gcaaaaaaat
     7021 aatcaaacaa aacctcatca ggcatggcaa aaatacaaat aaaatccgag aagctcactc
     7081 cttagcacgt ggccagcatc ttagcgtcgg tggttacaaa gaaaattcga caaactgtta
     7141 tttttctatc tatttatttg aattgtgagc ggataacaat tacctttgtc ggcaattgtg
     7201 agcggataac aattaaataa agatattctc gtcaaacaaa tataaataat ataaacatgg
     7261 ataagaaata ctcaataggc ttagctatcg gcacaaatag cgtcggatgg gcggtgatca
     7321 ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca
     7381 gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga
     7441 ctcgtctcaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc
     7501 tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg
     7561 aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata
     7621 tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat
     7681 tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga
     7741 ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg
     7801 acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta
     7861 acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat
     7921 tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca
     7981 ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg
     8041 ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa
     8101 ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac
     8161 tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga
     8221 ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac
     8281 aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt
     8341 atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa
     8401 aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc
     8461 aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta
     8521 ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa
     8581 aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt
     8641 ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg
     8701 tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc
     8761 ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata
     8821 acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag
     8881 gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta
     8941 agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag
     9001 gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta
     9061 aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa
     9121 cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc
     9181 tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt
     9241 tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt
     9301 ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt
     9361 tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg
     9421 aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa
     9481 aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg
     9541 aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga
     9601 aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg
     9661 aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca
     9721 tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatgccattg
     9781 ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata
     9841 aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact
     9901 attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga
     9961 aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg
    10021 ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta
    10081 aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat
    10141 tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc
    10201 atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc
    10261 caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga
    10321 ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata
    10381 tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc
    10441 taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca
    10501 cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga
    10561 caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc
    10621 gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt
    10681 cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag
    10741 agttactagg gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt
    10801 tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata
    10861 gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa
    10921 aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt
    10981 atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc
    11041 ataagcatta tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt
    11101 tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa
    11161 tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg
    11221 ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag
    11281 ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt
    11341 tgagtcagct aggaggtgac tgaattaatg cggctgcaat ttttttgggc ggggccgccc
    11401 aaaaaaatcc tagcaccctg cagcagtact gcttgaccat aagaacaaaa aaacttccga
    11461 taaagtttgg aagataaagc taaaagttct tatctttgca gtagcttacg ccaccctgtt
    11521 ccgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
    11581 gtggcaccga gtcggtgctt tttttctaga tgatgattaa ggatcttgcg tacaatttga
    11641 cgatcgtttc acagcatttg tgcattggct gcaacaa
//
