LOCUS       Exported                 779 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  Plant Gene expression Vector pSIM24 containing eGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    pSiM24-eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Sahoo DK, Dey N, Maiti IB
  TITLE     pSiM24 is a novel versatile gene expression vector for transient 
            assays as well as stable expression of foreign genes in plants.
  JOURNAL   PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988.
            eCollection 2014.
  PUBMED    24897541
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(41..760)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(44..760)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(86..107)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     435..454
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     694..715
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aaccgatgat acgaacgaaa gctctagact gcaggagctc ttacttgtac agctcgtcca
       61 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      121 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
      181 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      241 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
      301 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
      361 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
      421 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
      481 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
      541 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
      601 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
      661 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
      721 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ctcgagggat ccaagctta
//
